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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD66c Purified
Anti-Hu CD66c Purified
Regulatory status
RUO
Antigen
CD66c
Clone
B6.2
Format
Purified
Reactivity
Human
Application
IP, FC (QC tested)
Variant
0.1 mg
11-863-C100
In stock
158.40 USD
Variant
0.1 mg
11-863-C100
In stock
158.40 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody B6.2 recognizes a conformationally dependent epitope of native CD66c, a GPI-anchored extracellular glycoprotein expressed on granulocytes and epithelial cells.
Workshop
HLDA V: MA135, HLDA VI: MA86
Application
IP, FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 3-12 µg/ml
Reactivity
Human
Immunogen
Extracts from human breast carcinoma cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CEAL, NCA, CEACAM6
Antigen description
CD66c is a GPI-anchored glycoprotein capable of homophilic adhesion and heterophilic binding to CD66a-e, CD62E, and galectins. It is expressed on granulocytes and epithelial cells, and has potential applications in the detection of sites of infection and inflammation.
Entrez Gene ID
4680
UniProt ID
P40199
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD66c (B6.2) purified antibody (concentration in sample 3 μg/ml, GAM APC).
Separation of neutrophil granulocytes stained anti-human CD66c (B6.2) purified antibody (concentration in sample 3 μg/ml, GAM APC, red-filled) from neutrophil granulocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Product specific references:
Szpak CA, Johnston WW, Lottich SC, Kufe D, Thor A, Schlom J: Patterns of reactivity of four novel monoclonal antibodies (B72.3, DF3, B1.1 and B6.2) with cells in human malignant and benign effusions. Acta Cytol. 1984 Jul-Aug;28(4):356-67.
PubMed
Yamagami S, Yokoo S, Amano S, Ebihara N: Characterization of bone marrow derived cells in the substantia propria of the human conjunctiva. Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4476-81.
PubMed
Watt SM, Teixeira AM, Zhou GQ, Doyonnas R, Zhang Y, Grunert F, Blumberg RS, Kuroki M, Skubitz KM, Bates PA: Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site. Blood. 2001 Sep 1;98(5):1469-79.
PubMed
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K: Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow. J Biol Chem. 2008 Jun 6;283(23):15647-55.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-863-C100
In stock
158.40 USD
Variant
0.1 mg
11-863-C100
In stock
158.40 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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