Anti-Hu CD65 Purified

Anti-Hu CD65 Purified
Regulatory status
RUO
Antigen
CD65
Clone
VIM8
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-793-C100
Ask for delivery term HERE
130.00 USD

0.025 mg
11-793-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).
Workshop
HLDA II: WS Code M 27; HLDA III: WS Code M 261; HLDA IV: WS Code M 23; HLDA V: WS Code MA095, MA096; HLDA VI: WS Code MR12
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity
Human
Immunogen
THP-1 cell line
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ceramide-dodecasaccharide, type II fucoganglioside
Antigen description
CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.
11-793_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD65 (VIM8) purified antibody (concentration in sample 5 μg/ml, GAM APC).
11-793_FC_Histogram
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD65 (VIM8) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Product specific references:

Kniep B, Peter-Katalinic J, Müthing J, Majdic O, Pickl WF, Knapp W: The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer. J Biochem. 1996 Mar;119(3):456-62.
PubMed
Paietta E, Neuberg D, Bennett JM, Dewald G, Rowe JM, Cassileth PA, Cripe L, Tallman MS, Wiernik PH and the Eastern Cooperative Oncology Group: Low expression of the myeloid differentiation antigen CD65s, a feature of poorly differentiated AML in older adults: study of 711 patients enrolled in ECOG trials. Leukemia. 2003 Aug;17(8):1544-50.
PubMed
Variant
0.1 mg
11-793-C100
Ask for delivery term HERE
130.00 USD

0.025 mg
11-793-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD