Anti-Hu CD65 FITC

Anti-Hu CD65 FITC
Regulatory status
RUO
Antigen
CD65
Clone
VIM8
Format
FITC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-793-T100
In stock

25 tests
1F-793-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgM
Specificity
The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on peripheral blood granulocytes (highly) and monocytes (weakly).
Workshop
HLDA II: WS Code M 27; HLDA III: WS Code M 261; HLDA IV: WS Code M 23; HLDA V: WS Code MA095, MA096; HLDA VI: WS Code MR12
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
THP-1 cell line
Other names
ceramide-dodecasaccharide, type II fucoganglioside
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1F-793 FC
Flow cytometry analysis (surface staining) of CD65 in human peripheral blood with anti-CD65 (VIM8) FITC.

Product specific references:

Kniep B, Peter-Katalinic J, Müthing J, Majdic O, Pickl WF, Knapp W: The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer. J Biochem. 1996 Mar;119(3):456-62.
PubMed
Paietta E, Neuberg D, Bennett JM, Dewald G, Rowe JM, Cassileth PA, Cripe L, Tallman MS, Wiernik PH and the Eastern Cooperative Oncology Group: Low expression of the myeloid differentiation antigen CD65s, a feature of poorly differentiated AML in older adults: study of 711 patients enrolled in ECOG trials. Leukemia. 2003 Aug;17(8):1544-50.
PubMed
Variant
100 tests
1F-793-T100
In stock

25 tests
1F-793-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock