Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD63 Alexa Fluor<sup>®</sup> 647

Anti-Hu CD63 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CD58

Clone

MEM-63

Format

Alexa Fluor^® 647

Reactivity

Pig, Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

A6-343-T100

In stock

248.60 USD

Variant

100 tests

A6-343-T100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa extracellular membrane glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.

Workshop

HLDA VI: WS Code AS A047

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Reactivity

Pig, Human

Immunogen

NALM-6 human pre-B cell line

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

LFA3, AG3

Antigen description

CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.

Entrez Gene ID 965

UniProt ID P19256

Flow cytometry surface staining pattern of human IgE stimulated peripheral whole blood stained using anti-human CD63 (MEM-63) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of anti-IgE stimulated human peripheral blood mononuclear cells stained using anti-human CD203c (NP4D6) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD63 (MEM-63) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD203c positive CD63 positive basophils (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of anti-IgE stimulated human peripheral whole blood stained using anti-human CD63 (MEM-63) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14;12(26):4237-40.
PubMed

Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun;16(6):667-73.
PubMed

Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G; I-BFM-ALL-FCM-MRD-Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov;88(11):1245-52.
PubMed

Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2-CD58 complex. Biophys J. 2003 Apr;84(4):2223-33.
PubMed