Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD62P Purified

Anti-Hu CD62P Purified

Regulatory status

RUO

Antigen

CD62P

Clone

HI62P

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested)

Variant

0.1 mg

11-450-C100

In stock

158.40 USD

0.025 mg

11-450-C025

Delivery 1 week

79.20 USD

Variant

0.1 mg

11-450-C100

In stock

158.40 USD

0.025 mg

11-450-C025

Delivery 1 week

79.20 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody HI62P recognizes an extracellular epitope of CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

Application

WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Human

Immunogen

Human platelets

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

P-selectin, GMP140, SELP, PADGEM, GRMP, LECAM3, PSEL

Antigen description

CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.

Entrez Gene ID 6403

UniProt ID P16109

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD62P (HI62P) purified antibody (concentration in sample 0.56 μg/ml).

Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62P (HI62P) purified antibody (concentration in sample 0.56 μg/ml).

Anti-Hu CD62P Purified (clone HI62P) works in WB application under reducing conditions.

Western blotting analysis was performed on RIPA buffer extracts of thrombocytes, leukocytes, and Jurkat cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody HI62P (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.

CD62P was detected at ~ 125 kDa in thrombocytes and leukocytes.

General references:

Harakawa N, Shigeta A, Wato M, Merrill-Skoloff G, Furie BC, Furie B, Okazaki T, Domae N, Miyasaka M, Hirata T. P-selectin glycoprotein ligand-1 mediates L-selectin-independent leukocyte rolling in high endothelial venules of peripheral lymph nodes. Int Immunol. 2007 Mar;19(3):321-9.
PubMed

Martinez M, Joffraud M, Giraud S, Baïsse B, Bernimoulin MP, Schapira M, Spertini O: Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII. J Biol Chem. 2005 Feb 18;280(7):5378-90.
PubMed

Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13519-24.
PubMed

Ramachandran V, Yago T, Epperson TK, Kobzdej MM, Nollert MU, Cummings RD, Zhu C, McEver RP: Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10166-71.
PubMed

Product specific references:

Marques P, Collado A, Martinez-Hervás S, Domingo E, Benito E, Piqueras L, Real JT, Ascaso JF, Sanz MJ: Systemic Inflammation in Metabolic Syndrome: Increased Platelet and Leukocyte Activation, and Key Role of CX3CL1/CX3CR1 and CCL2/CCR2 Axes in Arterial Platelet-Proinflammatory Monocyte Adhesion. J Clin Med. 2019 May 18;8(5). pii: E708.
PubMed

Chiva-Blanch G, Laake K, Myhre P, Bratseth V, Arnesen H, Solheim S, Badimon L, Seljeflot I: High Adherence to the Nordic Diet Is Associated with Lower Levels of Total and Platelet-Derived Circulating Microvesicles in a Norwegian Population. Nutrients. 2019 May 18;11(5). pii: E1114.
PubMed