Anti-Hu CD62P FITC

Anti-Hu CD62P FITC
Regulatory status
RUO
Antigen
CD62P
Clone
AK4
Format
FITC
Reactivity
Human, Non-human primates
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-667-T100
In stock

25 tests
1F-667-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The antibody AK4 recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
Workshop
HLDA VI: WS Code P-44
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Immunogen
Human platelets
Other names
P-selectin, GMP140, SELP, PADGEM, GRMP, LECAM3, PSEL
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1F-667 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD62P (AK4) FITC.

General references:

Harakawa N, Shigeta A, Wato M, Merrill-Skoloff G, Furie BC, Furie B, Okazaki T, Domae N, Miyasaka M, Hirata T. P-selectin glycoprotein ligand-1 mediates L-selectin-independent leukocyte rolling in high endothelial venules of peripheral lymph nodes. Int Immunol. 2007 Mar;19(3):321-9.
PubMed
Martinez M, Joffraud M, Giraud S, Baïsse B, Bernimoulin MP, Schapira M, Spertini O: Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII. J Biol Chem. 2005 Feb 18;280(7):5378-90.
PubMed
Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13519-24.
PubMed
Ramachandran V, Yago T, Epperson TK, Kobzdej MM, Nollert MU, Cummings RD, Zhu C, McEver RP: Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow. Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10166-71.
PubMed

Product specific references:

Skinner MP, Lucas CM, Burns GF, Chesterman CN, Berndt MC: GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991 Mar 25;266(9):5371-4.
PubMed
Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol. 2004 Mar;122(3):830-6.
PubMed
Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets. Br J Haematol. 1999 Feb;104(2):220-9.
PubMed
Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB J. 1998 Jan;12(1):79-89.
PubMed
Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J Exp Med. 1992 Apr 1;175(4):1147-50.
PubMed
Skinner MP, Lucas CM, Burns GF, Chesterman CN, Berndt MC: GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991 Mar 25;266(9):5371-4.
PubMed
Variant
100 tests
1F-667-T100
In stock

25 tests
1F-667-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock