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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD62L Alexa Fluor<sup>®</sup> 647
Anti-Hu CD62L Alexa Fluor
®
647
Regulatory status
RUO
Antigen
CD62L
Clone
DREG56
Format
Alexa Fluor
®
647
Reactivity
Human
Application
FC (QC tested), IHC-F
Excitation laser
red (633 nm)
Variant
100 tests
A6-630-T100
In stock
242.00 USD
Variant
100 tests
A6-630-T100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.
Workshop
HLDA V: WS Code S056
Application
FC (QC tested), IHC-F
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
PMA-activated human peripheral blood leukocytes
Preparation
Purified antibody is conjugated with Alexa Fluor
®
647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR
Antigen description
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Entrez Gene ID
6402
UniProt ID
P14151
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD62L (DREG56) Alexa Fluor® 647 antibody (concentration in sample 1 μg/ml).
Separation of human neutrophil granulocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) Alexa Fluor® 647 antibody (concentration in sample 1 μg/ml).
General references:
Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13519-24.
PubMed
Ding Z, Issekutz TB, Downey GP, Waddell TK: L-selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003 Jun 1;101(11):4245-52.
PubMed
Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L-selectin (CD62L) cross-linking signals neutrophil adhesive functions via the Mac-1 (CD11b/CD18) beta 2-integrin. J Immunol. 1995 Aug 1;155(3):1502-14.
PubMed
von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC. L-selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX-dependent and -independent recognition pathways. Blood. 1993 Jul 1;82(1):182-91.
PubMed
Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct;263(4 Pt 2):H1034-44.
PubMed
Product specific references:
Abraham WM, Ahmed A, Sabater JR, Lauredo IT, Botvinnikova Y, Bjercke RJ, Hu X, Revelle BM, Kogan TP, Scott IL, Dixon RA, Yeh ET, Beck PJ: Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep. Am J Respir Crit Care Med. 1999 Apr;159(4 Pt 1):1205-14.
PubMed
Xu T, Chen L, Shang X, Cui L, Luo J, Chen C, Ba X, Zeng X: Critical role of Lck in L-selectin signaling induced by sulfatides engagement. J Leukoc Biol. 2008 Oct;84(4):1192-201.
PubMed
Killcock DJ, Parsons M, Zarrouk M, Ameer-Beg SM, Ridley AJ, Haskard DO, Zvelebil M, Ivetic A: In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin. J Biol Chem. 2009 Mar 27;284(13):8833-45.
PubMed
Tu W, Mao H, Zheng J, Liu Y, Chiu SS, Qin G, Chan PL, Lam KT, Guan J, Zhang L, Guan Y, Yuen KY, Peiris JS, Lau YL: Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus. J Virol. 2010 Jul;84(13):6527-35.
PubMed
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Jutila MA, Kurk S, Jackiw L, Knibbs RN, Stoolman LM: L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts. J Immunol. 2002 Aug 15;169(4):1768-73.
PubMed
Kishimoto TK, Jutila MA, Butcher EC: Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc Natl Acad Sci U S A. 1990 Mar;87(6):2244-8.
PubMed
Kishimoto TK, Warnock RA, Jutila MA, Butcher EC, Lane C, Anderson DC, Smith CW: Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro. Blood. 1991 Aug 1;78(3):805-11.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
A6-630-T100
In stock
242.00 USD
Variant
100 tests
A6-630-T100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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