Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD62E PE

Anti-Hu CD62E PE

Regulatory status

RUO

Antigen

CD62E

Clone

HAE-1f

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-838-T100

In stock

242.00 USD

Variant

100 tests

1P-838-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal HAE-1f recognizes an extracellular epitope of CD62E, a transmembrane glycoprotein expressed on activated endothelial cells, as well as in skin, placenta, and bone marrow endothelium.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

TNF-activated HUVEC and human CD40 cDNA-transfected cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

E-selectin, ELAM1, ESEL, SELE

Antigen description

CD62E (E-selectin) is a type I transmembrane glycoprotein of 97 - 115 kDa. It recognizes carbohydrate ligands on various molecules, and promotes leukocyte extravasation by mediating leukocyte rolling on activated endothelia during inflammation. CD62E is expressed above all in inflammated endothelium, but it can also be found on resting endothelium in certain organs, such as skin, or placenta.

Entrez Gene ID 6401

UniProt ID P16581

Separation of TNF-alpha stimulated HUVEC cells stained using anti-CD62E (HAE-1f) PE antibody (10 μl reagent / 100 μl of cell suspension, red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 8 μg/ml, same as CD62E PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HUVEC cell suspension.

Product specific references:

Tu L, Chen A, Delahunty MD, Moore KL, Watson SR, McEver RP, Tedder TF: L-selectin binds to P-selectin glycoprotein ligand-1 on leukocytes: interactions between the lectin, epidermal growth factor, and consensus repeat domains of the selectins determine ligand binding specificity. J Immunol. 1996 Nov 1;157(9):3995-4004.
PubMed

Trappenburg MC, van Schilfgaarde M, Marchetti M, Spronk HM, ten Cate H, Leyte A, Terpstra WE, Falanga A: Elevated procoagulant microparticles expressing endothelial and platelet markers in essential thrombocythemia. Haematologica. 2009 Jul;94(7):911-8.
PubMed