Anti-Hu CD62E PE

Anti-Hu CD62E PE
Regulatory status
RUO
Antigen
CD62E
Clone
HAE-1f
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-838-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal HAE-1f recognizes an extracellular epitope of CD62E, a transmembrane glycoprotein expressed on activated endothelial cells, as well as in skin, placenta, and bone marrow endothelium.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
TNF-activated HUVEC and human CD40 cDNA-transfected cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
E-selectin, ELAM1, ESEL, SELE
Antigen description
CD62E (E-selectin) is a type I transmembrane glycoprotein of 97 - 115 kDa. It recognizes carbohydrate ligands on various molecules, and promotes leukocyte extravasation by mediating leukocyte rolling on activated endothelia during inflammation. CD62E is expressed above all in inflammated endothelium, but it can also be found on resting endothelium in certain organs, such as skin, or placenta.
Entrez Gene ID 6401
UniProt ID P16581
1P-838_FC_Histogram
Separation of TNF-alpha stimulated HUVEC cells stained using anti-CD62E (HAE-1f) PE antibody (10 μl reagent / 100 μl of cell suspension, red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 8 μg/ml, same as CD62E PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HUVEC cell suspension.

Product specific references:

Tu L, Chen A, Delahunty MD, Moore KL, Watson SR, McEver RP, Tedder TF: L-selectin binds to P-selectin glycoprotein ligand-1 on leukocytes: interactions between the lectin, epidermal growth factor, and consensus repeat domains of the selectins determine ligand binding specificity. J Immunol. 1996 Nov 1;157(9):3995-4004.
PubMed
Trappenburg MC, van Schilfgaarde M, Marchetti M, Spronk HM, ten Cate H, Leyte A, Terpstra WE, Falanga A: Elevated procoagulant microparticles expressing endothelial and platelet markers in essential thrombocythemia. Haematologica. 2009 Jul;94(7):911-8.
PubMed
Variant
100 tests
1P-838-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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