Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD61 Purified

Anti-Hu CD61 Purified

Regulatory status

RUO

Antigen

CD61

Clone

VIPL2

Format

Purified

Reactivity

Human, Non-human primates

Application

WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-647-C100

In stock

143.00 USD

0.025 mg

11-647-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-647-C100

In stock

143.00 USD

0.025 mg

11-647-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody VIPL2 recognizes an extracellular epitope of CD61, a 90-110 kDa transmembrane glycoprotein of integrin family, expressed on platelets, megacaryocytes, osteoclasts, endothelial cells and other cell types, including leucocytes and smooth muscle cells.

Workshop

HLDA V: WS Code 5T-124

Application

WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human, Non-human primates

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

integrin beta 3, ITGB3, GP3A

Antigen description

CD61 (beta3 integrin) is a transmembrane glycoprotein, which associates with CD41 or CD51 molecules to form heterodimeric adhesion receptores. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. It binds to fibronectin, fibrinogen and von Willebrand factor, and is involved in platelet aggregation. CD51/CD61 complex has similar binding properties and is involved in modulating migration and survival of angiogenic endothelial cells.

Entrez Gene ID 3690

UniProt ID P05106

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD61 (VIPL2) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Separation of human CD61 positive thrombocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD61 (VIPL2) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Product specific references:

Roberts MS, Woods AJ, Dale TC, Van Der Sluijs P, Norman JC: Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins. Mol Cell Biol. 2004 Feb;24(4):1505-15.
PubMed

Barrett L, Dai C, Gamberg J, Gallant M, Grant M: Circulating CD14-CD36+ peripheral blood mononuclear cells constitutively produce interleukin-10. J Leukoc Biol. 2007 Jul;82(1):152-60.
PubMed

Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK: VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment. J Virol. 2002 Feb;76(3):1109-23.
PubMed

Williams CA, Mondal D, Agrawal KC: The HIV-1 Tat protein enhances megakaryocytic commitment of K562 cells by facilitating CREB transcription factor coactivation by CBP. Exp Biol Med (Maywood). 2005 Dec;230(11):872-84.
PubMed

Mondal D, Williams CA, Ali M, Eilers M, Agrawal KC: The HIV-1 Tat protein selectively enhances CXCR4 and inhibits CCR5 expression in megakaryocytic K562 cells. Exp Biol Med (Maywood). 2005 Oct;230(9):631-44.
PubMed

Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, Buck DW, Schmitz J: BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol. 2000 Dec 1;165(11):6037-46.
PubMed