Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD59 Purified

Anti-Hu CD59 Purified

Regulatory status

RUO

Antigen

CD59

Clone

MEM-43/5

Format

Purified

Reactivity

Mouse, Human

Application

IP, WB, FC (QC tested), IHC-P

Variant

0.1 mg

11-234-C100

In stock

143.00 USD

0.025 mg

11-234-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-234-C100

In stock

143.00 USD

0.025 mg

11-234-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

Workshop

HLDA V: WS Code AS S012

Application

IP, WB, FC (QC tested), IHC-P

Application details

Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: blood.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; positive control: mouse spleen, non-reducing conditions.

Reactivity

Mouse, Human

Immunogen

Thymocytes and T lymphocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

Antigen description

CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.

Entrez Gene ID 966

UniProt ID P13987

Western blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43; cat. no. 11-233-C100)

Separation of human neutrophil granulocytes (red-filled) from human CD59 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD59 (MEM-43/5) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Immunohistochemistry staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5). Commercially tested by LifeSpan BioSciences.

General references:

Baalasubramanian S, Harris CL, Donev RM, Mizuno M, Omidvar N, Song WC, Morgan BP: CD59a is the primary regulator of membrane attack complex assembly in the mouse. J Immunol. 2004 Sep 15;173(6):3684-92.
PubMed

Menu E, Tsai BC, Bothwell AL, Sims PJ, Bierer BE: CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation. J Immunol. 1994 Sep 15;153(6):2444-56.
PubMed

Rooney IA, Davies A, Griffiths D, Williams JD, Davies M, Meri S, Lachmann PJ, Morgan BP: The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin Exp Immunol. 1991 Feb;83(2):251-6.
PubMed

Meri S, Morgan BP, Davies A, Daniels RH, Olavesen MG, Waldmann H, Lachmann PJ: Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology. 1990 Sep;71(1):1-9.
PubMed

Product specific references:

Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol. J Biol Chem. 1997 Aug 1;272(31):19242-7.
PubMed

Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
PubMed

Bodian DL, Davis SJ, Morgan BP, Rushmere NK: Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3;185(3):507-16.
PubMed

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).