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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD58 Biotin
Anti-Hu CD58 Biotin
Regulatory status
RUO
Antigen
CD58
Clone
MEM-63
Format
Biotin
Reactivity
Pig, Human
Application
FC (QC tested)
Variant
0.1 mg
1B-232-C100
In stock
198.00 USD
0.025 mg
1B-232-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-232-C100
In stock
198.00 USD
0.025 mg
1B-232-C025
Delivery 1 week
99.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa extracellular membrane glycoprotein distributed over many tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.
Workshop
HLDA VI: WS Code AS A047
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 2-12 μg/ml.
Reactivity
Pig, Human
Immunogen
NALM-6 human pre-B cell line
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LFA3, AG3
Antigen description
CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.
Entrez Gene ID
965
UniProt ID
P19256
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD58 (MEM-63) Biotin antibody (concentration in sample 1.67 μg/ml, Streptavidin APC).
Separation of human neutrophil granulocytes (red-filled) from CD58 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD58 (MEM-63) Biotin antibody (concentration in sample 1.67 μg/ml, Streptavidin APC).
General references:
Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14;12(26):4237-40.
PubMed
Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun;16(6):667-73.
PubMed
Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G; I-BFM-ALL-FCM-MRD-Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov;88(11):1245-52.
PubMed
Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2-CD58 complex. Biophys J. 2003 Apr;84(4):2223-33.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
1B-232-C100
In stock
198.00 USD
0.025 mg
1B-232-C025
Delivery 1 week
99.00 USD
Variant
0.1 mg
1B-232-C100
In stock
198.00 USD
0.025 mg
1B-232-C025
Delivery 1 week
99.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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