Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD57 Purified

Anti-Hu CD57 Purified

Regulatory status

RUO

Antigen

CD57

Clone

TB01

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested), IHC(F), IHC(P), ICC

Variant

0.1 mg

11-158-C100

In stock

143.00 USD

0.025 mg

11-158-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-158-C100

In stock

143.00 USD

0.025 mg

11-158-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.

Workshop

HLDA VI: WS Code NK16

Application

WB, FC (QC tested), IHC(F), IHC(P), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.

Reactivity

Human

Immunogen

A pool of neuroblastoma cell lines

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HNK1, LEU7, 3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc

Antigen description

CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.

Entrez Gene ID 27087

UniProt ID Q9P2W7

Flow cytometry analysis (surface staining) of human buffy coat cells with anti-human CD57 (TB01) purified, GAM-APC.

Immunohistochemistry staining of human adrenal gland (paraffin-embedded sections) with anti-CD57 (TB01), 10 μg/ml. Commercially tested by LifeSpan BioSciences.

Product specific references:

Lim SA, Kim TJ, Lee JE, Sonn CH, Kim K, Kim J, Choi JG, Choi IK, Yun CO, Kim JH, Yee C, Kumar V, Lee KM: Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013 Apr 15;73(8):2598-607.
PubMed

Cunha KS, Caruso AC, Faria PA, Silva LE, Fonseca EC, Geller M, de Moura-Neto RS, Lopes VS: Evaluation of Bcl-2, Bcl-x and cleaved caspase-3 in malignant peripheral nerve sheath tumors and neurofibromas. An Acad Bras Cienc. 2013;85(4):1497-511.
PubMed

Phadwal K, Alegre-Abarrategui J, Watson AS, Pike L, Anbalagan S, Hammond EM, Wade-Martins R, McMichael A, Klenerman P, Simon AK: A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells. Autophagy. 2012 Apr;8(4):677-89.
PubMed

Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson MA, Kekäläinen E, Johansson H, Mjösberg J, Westgren M, Lankisch TO, Wedemeyer H, Ellis EC, Ljunggren HG, Michaëlsson J, Björkström NK: Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015 Mar 15;194(6):2467-71.
PubMed

Wangerin H, Kristiansen G, Schlomm T, Stephan C, Gunia S, Zimpfer A, Weichert W, Sauter G, Erbersdobler A: CD57 expression in incidental, clinically manifest, and metastatic carcinoma of the prostate. Biomed Res Int. 2014;2014:356427.
PubMed

Fernandez S, French MA, Price P: Immunosenescent CD57+CD4+ T-cells accumulate and contribute to interferon-γ responses in HIV patients responding stably to ART. Dis Markers. 2011;31(6):337-42.
PubMed