Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD56 Purified

Anti-Hu CD56 Purified

Regulatory status

RUO

Antigen

CD56

Clone

LT56

Format

Purified

Reactivity

Human

Application

ELISA, WB, FC (QC tested)

Variant

0.1 mg

11-789-C100

In stock

130.00 USD

0.025 mg

11-789-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-789-C100

In stock

130.00 USD

0.025 mg

11-789-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody LT56 recognizes an extracellular epitope of CD56 (NCAM), a transmembrane glycoprotein expressed ubiquitously in the nervous system and found also on T cells and NK cells.

Workshop

HLDA X

Application

ELISA, WB, FC (QC tested)

Application details

Western blotting: Non-reducing conditions.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

Cell line KG1a

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

NCAM1, MSK39

Antigen description

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours.

Entrez Gene ID 4684

UniProt ID P13591

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD56 (LT56) purified antibody (concentration in sample 2 μg/ml, GAM APC).

Separation of human CD56 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD56 (LT56) purified antibody (concentration in sample 2 μg/ml, GAM APC).

General references:

McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. Int J Gynecol Pathol. 2007 Jul;26(3):322-7.
PubMed

Ohishi Y, Kaku T, Oya M, Kobayashi H, Wake N, Tsuneyoshi M: CD56 expression in ovarian granulosa cell tumors, and its diagnostic utility and pitfalls. Gynecol Oncol. 2007 Oct;107(1):30-8.
PubMed

Jakovcevski I, Mo Z, Zecevic N: Down-regulation of the axonal polysialic acid-neural cell adhesion molecule expression coincides with the onset of myelination in the human fetal forebrain. Neuroscience. 2007 Oct 26;149(2):328-37.
PubMed

Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991 Jun 15;146(12):4421-6.
PubMed

Product specific references:

Munteanu AN, Surcel M, Huică RI, Isvoranu G, Constantin C, Pîrvu IR, Chifiriuc C, Ulmeanu C, Ursaciuc C, Neagu M: Peripheral immune cell markers in children with recurrent respiratory infections in the absence of primary immunodeficiency. Exp Ther Med 2019, June 26, 1693-1700
PubMed

Variant

0.1 mg

11-789-C100

In stock

130.00 USD

0.025 mg

11-789-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-789-C100