Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD54 Purified Azide Free

Anti-Hu CD54 Purified Azide Free

Regulatory status

RUO

Antigen

CD54

Clone

1H4

Format

Purified Azide Free

Reactivity

Human

Application

WB, FC (QC tested), IHC(F), IHC(P), ICC

Variant

0.1 mg

10-429-C100

In stock

143.00 USD

0.025 mg

10-429-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

10-429-C100

In stock

143.00 USD

0.025 mg

10-429-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Application

WB, FC (QC tested), IHC(F), IHC(P), ICC

Application details

Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required.
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.

Reactivity

Human

Immunogen

Raji cells and spleen cells fused with NS1 cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ICAM-1, BB2, P3.58

Antigen description

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Entrez Gene ID 3383

UniProt ID P05362

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD54 (1H4) purified antibody (concentration in sample 3 μg/ml, GAM FITC).

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD54 (1H4) purified antibody (concentration in sample 3 μg/ml, GAM FITC).

Western blotting analysis of human CD54 using mouse monoclonal antibody 1H4 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD54 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88 kDa.

General references:

Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May;85(9):3095-9.
PubMed

Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 15;73(7):1896-903.
PubMed

Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425-34.
PubMed

Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 10;68(1):63-9. Erratum in: Cell 1992 Mar 6;68(5):following 994.
PubMed

Product specific references:

Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay-accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J Gen Virol. 2004 Mar;85(Pt 3):731-8.
PubMed