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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD54 Purified
Anti-Hu CD54 Purified
Regulatory status
RUO
Antigen
CD54
Clone
MEM-111
Format
Purified
Reactivity
Human, Cow, Rat
Application
ELISA, WB, FC (QC tested), IHC-P, ICC
Variant
0.1 mg
11-228-C100
In stock
147.40 USD
0.025 mg
11-228-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
11-228-C100
In stock
147.40 USD
0.025 mg
11-228-C025
Delivery 1 week
73.70 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
Workshop
HLDA VI: WS Code AS A049
Application
ELISA, WB, FC (QC tested), IHC-P, ICC
Application details
Flow cytometry: Recommended dilution: 0.5-4 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: thymus, RE cells.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.
Reactivity
Human, Cow, Rat
Immunogen
Raji human Burkitt's lymphoma cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ICAM-1, BB2, P3.58
Antigen description
CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Entrez Gene ID
3383
UniProt ID
P05362
Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD54 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88 kDa.
Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).
Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).
Western blotting analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111. Lower bands represent tubulin as a loading control.
General references:
Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May;85(9):3095-9.
PubMed
Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 15;73(7):1896-903.
PubMed
Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425-34.
PubMed
Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 10;68(1):63-9. Erratum in: Cell 1992 Mar 6;68(5):following 994.
PubMed
Product specific references:
Nagaraju K et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998).
PubMed
Tachimoto H. et al., Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases. Am J Respir Cell Mol Biol 26, 645 (2002).
PubMed
Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003).
PubMed
Hertel L et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003).
PubMed
Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9.
PubMed
Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36.
PubMed
Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9.
PubMed
Leeuwenberg JFM et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992).
PubMed
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-228-C100
In stock
147.40 USD
0.025 mg
11-228-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
11-228-C100
In stock
147.40 USD
0.025 mg
11-228-C025
Delivery 1 week
73.70 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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