Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD54 PE

Anti-Hu CD54 PE

Regulatory status

RUO

Antigen

CD54

Clone

MEM-111

Format

Reactivity

Human, Cow, Rat

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-228-T100

In stock

242.00 USD

25 tests

1P-228-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-228-T100

In stock

242.00 USD

25 tests

1P-228-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Workshop

HLDA VI: WS Code AS A049

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human, Cow, Rat

Immunogen

Raji human Burkitt's lymphoma cell line

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ICAM-1, BB2, P3.58

Antigen description

CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Entrez Gene ID 3383

UniProt ID P05362

Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE.

General references:

Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May;85(9):3095-9.
PubMed

Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 15;73(7):1896-903.
PubMed

Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425-34.
PubMed

Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 10;68(1):63-9. Erratum in: Cell 1992 Mar 6;68(5):following 994.
PubMed

Product specific references:

Nagaraju K et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998).
PubMed

Tachimoto H. et al., Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases. Am J Respir Cell Mol Biol 26, 645 (2002).
PubMed

Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003).
PubMed

Hertel L et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003).
PubMed

Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9.
PubMed

Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36.
PubMed

Leeuwenberg JFM et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992).
PubMed

Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).