Anti-Hu CD53 Pacific Blue™

Anti-Hu CD53 Pacific Blue™
Regulatory status
RUO
Antigen
CD53
Clone
MEM-53
Format
Pacific Blue™
Reactivity
Human
Application
FC (QC tested)
Excitation laser
violet (405 nm)
Variant
100 tests
PB-227-T100
Out of stock

25 tests
PB-227-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Workshop
HLDA IV: WS Code NL 59; HLDA V: WS Code B CD53.5; HLDA V: WS Code BP BP287; HLDA V: WS Code T T-096; HLDA V: WS Code X XB004
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Leukocytes of pacient suffering from a LGL-type leukemia.
Other names
Tetraspanin-25, MOX44, TSPAN25
Preparation
The purified antibody is conjugated with Pacific Blue™ under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an agreement between Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corporation), and Exbio Praha, a.s., and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by Molecular Probes, Inc. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity), including use in flow cytometry that does not utilize a bead based array, but excluding use in combination with microarrays or High Content Screening. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Molecular Probes, Inc., Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.

General references:

Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells. Kidney Int. 2003 Feb;63(2):534-42.
PubMed
Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003 Feb 27;22(8):1219-24.
PubMed
Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol Cells. 2004 Feb 29;17(1):125-31.
PubMed
Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. Int Immunol. 2002 Mar;14(3):249-58.
PubMed
Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. J Leukoc Biol. 1998 Jun;63(6):699-706.
PubMed
Angelisova P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics. 1990;32(4):281-5.
PubMed
Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993 Jul 15;151(2):707-16.
PubMed

Product specific references:

Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed
Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13.
PubMed
Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol. 1996 Oct 1;157(7):2939-46.
PubMed
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol. 1994 Dec 1;153(11):4997-5007.
PubMed
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97.
PubMed
Variant
100 tests
PB-227-T100
Out of stock

25 tests
PB-227-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock