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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD53 Pacific Blue™
Anti-Hu CD53 Pacific Blue™
Regulatory status
RUO
Antigen
CD53
Clone
MEM-53
Format
Pacific Blue™
Reactivity
Human
Application
FC (QC tested)
Excitation laser
violet (405 nm)
Variant
100 tests
PB-227-T100
In stock
242.00 USD
25 tests
PB-227-T025
Delivery 1 week
121.00 USD
Variant
100 tests
PB-227-T100
In stock
242.00 USD
25 tests
PB-227-T025
Delivery 1 week
121.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Workshop
HLDA IV: WS Code NL 59; HLDA V: WS Code B CD53.5; HLDA V: WS Code BP BP287; HLDA V: WS Code T T-096; HLDA V: WS Code X XB004
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Leukocytes of pacient suffering from a LGL-type leukemia.
Preparation
Purified antibody is conjugated with Pacific Blue™ NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
Tetraspanin-25, MOX44, TSPAN25
Antigen description
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Entrez Gene ID
963
UniProt ID
P19397
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD53 (MEM-53) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
Separation of human monocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD53 (MEM-53) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
General references:
Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells. Kidney Int. 2003 Feb;63(2):534-42.
PubMed
Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003 Feb 27;22(8):1219-24.
PubMed
Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol Cells. 2004 Feb 29;17(1):125-31.
PubMed
Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics. Int Immunol. 2002 Mar;14(3):249-58.
PubMed
Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down-regulates CD53 cell surface antigen. J Leukoc Biol. 1998 Jun;63(6):699-706.
PubMed
Angelisova P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics. 1990;32(4):281-5.
PubMed
Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993 Jul 15;151(2):707-16.
PubMed
Product specific references:
Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed
Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13.
PubMed
Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol. 1996 Oct 1;157(7):2939-46.
PubMed
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol. 1994 Dec 1;153(11):4997-5007.
PubMed
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
PB-227-T100
In stock
242.00 USD
25 tests
PB-227-T025
Delivery 1 week
121.00 USD
Variant
100 tests
PB-227-T100
In stock
242.00 USD
25 tests
PB-227-T025
Delivery 1 week
121.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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