Anti-Hu CD52 Purified

Anti-Hu CD52 Purified
Regulatory status
RUO
Antigen
CD52
Clone
HI186
Format
Purified
Reactivity
Human
Application
FC (QC tested), IHC(P)
Variant
0.1 mg
11-368-C100
In stock

0.025 mg
11-368-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG2b
Specificity
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Workshop
HLDA VI: WS Code BP 523; HLDA VI: WS Code T 6T-057
Application
FC (QC tested), IHC(P)
Reactivity
Human
Immunogen
Human tonsil
Other names
CAMPATH-1, HE5, ESPE5
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-368 FC
Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE). Fig. 1A – CD52 negative pro T ALL (gate leukemic cells) Fig. 1B – CD52 positive cALL (gate leukemic cells)

General references:

Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C: Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15;274(42):29862-73.
PubMed
Treumann A, Lifely MR, Schneider P, Ferguson MA: Primary structure of CD52. J Biol Chem. 1995 Mar 17;270(11):6088-99.
PubMed
Rowan WC, Hale G, Tite JP, Brett SJ: Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan;7(1):69-77.
PubMed
Koyama K, Ito K, Hasegawa A: Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 2007;63:103-10.
PubMed
Domagała A, Kurpisz M: CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr;7(2):325-31.
PubMed
Variant
0.1 mg
11-368-C100
In stock

0.025 mg
11-368-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock