Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD52 Purified

Anti-Hu CD52 Purified

Regulatory status

RUO

Antigen

CD52

Clone

HI186

Format

Purified

Reactivity

Human

Application

FC (QC tested), IHC-P

Variant

0.1 mg

11-368-C100

In stock

165.00 USD

0.025 mg

11-368-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-368-C100

In stock

165.00 USD

0.025 mg

11-368-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.

Workshop

HLDA VI: WS Code BP 523; HLDA VI: WS Code T 6T-057

Application

FC (QC tested), IHC-P

Application details

Flow cytometry: Recommended dilution: 2 μg/ml.

Reactivity

Human

Immunogen

Human tonsil

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CAMPATH-1, HE5, ESPE5

Antigen description

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.

Entrez Gene ID 1043

UniProt ID P31358

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD52 (HI186) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Separation of human CD52 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD52 (HI186) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE). Fig. 1A – CD52 negative pro T ALL (gate leukemic cells) Fig. 1B – CD52 positive cALL (gate leukemic cells)

General references:

Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C: Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15;274(42):29862-73.
PubMed

Treumann A, Lifely MR, Schneider P, Ferguson MA: Primary structure of CD52. J Biol Chem. 1995 Mar 17;270(11):6088-99.
PubMed

Rowan WC, Hale G, Tite JP, Brett SJ: Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan;7(1):69-77.
PubMed

Koyama K, Ito K, Hasegawa A: Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 2007;63:103-10.
PubMed

Domagała A, Kurpisz M: CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr;7(2):325-31.
PubMed