Anti-Hu CD52 PE

Anti-Hu CD52 PE
Regulatory status
RUO
Antigen
CD52
Clone
HI186
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-368-T100
In stock
242.00 USD

25 tests
1P-368-T025
In stock
121.00 USD
Variant
100 tests
1P-368-T100
In stock
242.00 USD

25 tests
1P-368-T025
In stock
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Workshop
HLDA VI: WS Code BP 523; HLDA VI: WS Code T 6T-057
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human tonsil
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CAMPATH-1, HE5, ESPE5
Antigen description
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
Entrez Gene ID 1043
UniProt ID P31358
1P-368 FC
Flow cytometry analysis (surface staining) of CD52 in human peripheral blood with anti-CD52 (HI186) PE.

General references:

Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C: Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15;274(42):29862-73.
PubMed
Treumann A, Lifely MR, Schneider P, Ferguson MA: Primary structure of CD52. J Biol Chem. 1995 Mar 17;270(11):6088-99.
PubMed
Rowan WC, Hale G, Tite JP, Brett SJ: Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan;7(1):69-77.
PubMed
Koyama K, Ito K, Hasegawa A: Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 2007;63:103-10.
PubMed
Domagała A, Kurpisz M: CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr;7(2):325-31.
PubMed
Variant
100 tests
1P-368-T100
In stock
242.00 USD

25 tests
1P-368-T025
In stock
121.00 USD
Variant
100 tests
1P-368-T100
In stock
242.00 USD

25 tests
1P-368-T025
In stock
121.00 USD

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