Anti-Hu CD52 FITC

Anti-Hu CD52 FITC
Regulatory status
RUO
Antigen
CD52
Clone
4C8
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-878-T100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG3
Specificity
The mouse monoclonal antibody 4C8 recognizes CD52, a GPI-anchored glycopeptide expressed on the surface of lymphocytes, macrophages, epithelial cells of male reproductive tract, and mature sperms.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human lymphocytes
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CAMPATH-1, HE5, ESPE5
Antigen description
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
Entrez Gene ID 1043
UniProt ID P31358
1F-878_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD52 (4C8) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
1F-878_FC_Histogram
Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD52 (4C8) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C: Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15;274(42):29862-73.
PubMed
Treumann A, Lifely MR, Schneider P, Ferguson MA: Primary structure of CD52. J Biol Chem. 1995 Mar 17;270(11):6088-99.
PubMed
Rowan WC, Hale G, Tite JP, Brett SJ: Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan;7(1):69-77.
PubMed
Koyama K, Ito K, Hasegawa A: Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 2007;63:103-10.
PubMed
Domagała A, Kurpisz M: CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr;7(2):325-31.
PubMed

Product specific references:

Masuyama J, Kaga S, Kano S, Minota S: A novel costimulation pathway via the 4C8 antigen for the induction of CD4+ regulatory T cells. J Immunol. 2002 Oct 1;169(7):3710-6.
PubMed
Masuyama J, Yoshio T, Suzuki K, Kitagawa S, Iwamoto M, Kamimura T, Hirata D, Takeda A, Kano S, Minota S: Characterization of the 4C8 antigen involved in transendothelial migration of CD26(hi) T cells after tight adhesion to human umbilical vein endothelial cell monolayers. J Exp Med. 1999 Mar 15;189(6):979-90.
PubMed
Toh BH, Kyaw T, Tipping P, Bobik A: Immune Regulation by CD52-expressing CD4 T Cells. Cell Mol Immunol. 2013 Sep;10(5):379-82.
PubMed
Watanabe T, Masuyama J, Sohma Y, Inazawa H, Horie K, Kojima K, Uemura Y, Aoki Y, Kaga S, Minota S, Tanaka T, Yamaguchi Y, Kobayashi T, Serizawa I: CD52 is a novel costimulatory molecule for induction of CD4+ regulatory T cells. Clin Immunol. 2006 Sep;120(3):247-59.
PubMed
Variant
100 tests
1F-878-T100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD