Anti-Hu CD51/CD61 Purified

Anti-Hu CD51/CD61 Purified
Regulatory status
RUO
Antigen
CD51/CD61
Clone
23C6
Format
Purified
Reactivity
Human, Chicken
Application
IP, FC (QC tested), IHC(F)
Variant
0.1 mg
11-847-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 23C6 recognizes an extracellular epitope on CD51/CD61 complex, that is expressed mainly on human osteoclasts, but also e.g. on placenta, or melanoma cell lines. The epitope is native and sensitive to fixation. In chicken this antibody can be used to selectively identify the thrombocytes.
Workshop
HLDA IV
Application
IP, FC (QC tested), IHC(F)
Reactivity
Human, Chicken
Negative species
Rabbit, Pig, Cow
Immunogen
A cell suspension containing osteoclasts from osteoclastomas
Other names
VNRA, MSK8, ITGB3, ITGAV, Integrin alpha5beta3, OFA, VR
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-847_FC_Histogram
Separation of MCF-7 cells stained using anti-CD51/CD61 (23c3) purified antibody (concentration in sample 1,7 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Viertlboeck BC, Göbel TW: Chicken thrombocytes express the CD51/CD61 integrin. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):137-41
PubMed
Yamamoto T, Kurihara N, Yamaoka K, Ozono K, Okada M, Yamamoto K, Matsumoto S, Michigami T, Ono J, Okada S: Bone marrow-derived osteoclast-like cells from a patient with craniometaphyseal dysplasia lack expression of osteoclast-reactive vacuolar proton pump. J Clin Invest. 1993 Jan;91(1):362-7.
PubMed
Leukocyte Typing IV., Knapp WB. et al. (Eds.), Oxford University Press (1989).
Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M: The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol. 1989 Oct;109(4 Pt 1):1817-26.
PubMed
Barua A, Bitterman P, Bahr JM, Bradaric MJ, Hales DB, Luborsky JL, Abramowicz JS: Detection of tumor-associated neoangiogenesis by Doppler ultrasonography during early-stage ovarian cancer in laying hens: a preclinical model of human spontaneous ovarian cancer. J Ultrasound Med. 2010 Feb;29(2):173-82.
PubMed
Variant
0.1 mg
11-847-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD