Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD51/CD61 Alexa Fluor<sup>®</sup> 647

Anti-Hu CD51/CD61 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CD51/CD61

Clone

23C6

Format

Alexa Fluor^® 647

Reactivity

Human, Chicken

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

A6-847-T100

In stock

248.60 USD

Variant

100 tests

A6-847-T100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 23C6 recognizes an extracellular epitope on CD51/CD61 complex, that is expressed mainly on human osteoclasts, but also e.g. on placenta, or melanoma cell lines. The epitope is native and sensitive to fixation. In chicken this antibody can be used to selectively identify the thrombocytes.

Workshop

HLDA IV

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human, Chicken

Negative species

Rabbit, Pig, Cow

Immunogen

A cell suspension containing osteoclasts from osteoclastomas

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

VNRA, MSK8, ITGB3, ITGAV, Integrin alpha5beta3, OFA, VR

Antigen description

CD51/CD61 (integrin alpha5beta3), also known as osteoclast functional antigen, serves as a vitronectin receptor, and binds also to fibronectin, fibrinogen, thrombospondin, osteopontin, collagen, and von Willebrand factor. Expression of this antigen increases with melanoma progression. In healthy individuals CD51/CD61 is expressed mainly on osteoclasts, placenta, and endothelial cells, at lower levels on platelets and macrophages.

Separation of SK-MEL-30 cells stained using anti-human CD51/CD61 (23C6) Alexa Fluor® 647 antibody (concentration in sample 1.67 μg/ml, red-filled) from SK-MEL-30 cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 1.67 μg/ml, same as CD51/CD61 Alexa Fluor® 647 concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Viertlboeck BC, Göbel TW: Chicken thrombocytes express the CD51/CD61 integrin. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):137-41
PubMed

Yamamoto T, Kurihara N, Yamaoka K, Ozono K, Okada M, Yamamoto K, Matsumoto S, Michigami T, Ono J, Okada S: Bone marrow-derived osteoclast-like cells from a patient with craniometaphyseal dysplasia lack expression of osteoclast-reactive vacuolar proton pump. J Clin Invest. 1993 Jan;91(1):362-7.
PubMed

Leukocyte Typing IV., Knapp WB. et al. (Eds.), Oxford University Press (1989).

Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M: The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol. 1989 Oct;109(4 Pt 1):1817-26. (IHC-F, IP, WB)
PubMed

Barua A, Bitterman P, Bahr JM, Bradaric MJ, Hales DB, Luborsky JL, Abramowicz JS: Detection of tumor-associated neoangiogenesis by Doppler ultrasonography during early-stage ovarian cancer in laying hens: a preclinical model of human spontaneous ovarian cancer. J Ultrasound Med. 2010 Feb;29(2):173-82. (IHC)
PubMed