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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD50 Purified Low Endotoxin
Anti-Hu CD50 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD50
Clone
MEM-04
Format
Purified Low Endotoxin
Reactivity
Human
Application
FC (QC tested), IHC-P, FUNC
Variant
0.1 mg
12-452-C100
In stock
147.40 USD
0.025 mg
12-452-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
12-452-C100
In stock
147.40 USD
0.025 mg
12-452-C025
Delivery 1 week
73.70 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b kappa
Specificity
The antibody MEM-04 reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Application
FC (QC tested), IHC-P, FUNC
Application details
Functional application: The antibody MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: tonsil.
Reactivity
Human
Immunogen
Leukocytes of a patient suffering from LGL-type leukemia.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ICAM-3, ICAM-R
Antigen description
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
Entrez Gene ID
3385
UniProt ID
P32942
Separation of leukocytes stained using anti-human CD50 (MEM-04) purified antibody (concentration in sample 3 μg/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD50 (MEM-04) purified antibody (concentration in sample 3 μg/ml, GAM APC).
Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04). Commercially tested by LifeSpan BioSciences.
General references:
Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernández-Caselles T, Craig A, Pulido R, Sánchez-Madrid F. ICAM-3 interacts with LFA-1 and regulates the LFA-1/ICAM-1 cell adhesion pathway. J Cell Biol. 1993 Nov;123(4):1007-16.
PubMed
Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM-3, the third LFA-1 counterreceptor, is a co-stimulatory molecule for both resting and activated T lymphocytes. Eur J Immunol. 1993 Nov;23(11):2799-806.
PubMed
Arroyo AG, Campanero MR, Sánchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sánchez-Madrid F: Induction of tyrosine phosphorylation during ICAM-3 and LFA-1-mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J Cell Biol. 1994 Sep;126(5):1277-86.
PubMed
Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007 Mar;17(3):324-33.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAb_SDS_v1_GB.pdf
MPAb_SDS_v1_TR.pdf
MPAb_SDS_v6_AT.pdf
MPAb_SDS_v6_CH.pdf
MPAb_SDS_v6_CS.pdf
MPAb_SDS_v6_EN.pdf
MPAb_SDS_v6_ES.pdf
MPAb_SDS_v6_FR.pdf
MPAb_SDS_v6_IT.pdf
MPAb_SDS_v6_NO.pdf
MPAb_SDS_v6_PL.pdf
MPAb_SDS_v6_PT.pdf
MPAb_SDS_v6_SE.pdf
MPAb_SDS_v6_SK.pdf
MPAb_SDS_v6_SL.pdf
MPAb_SDS_v7_DE.pdf
Variant
0.1 mg
12-452-C100
In stock
147.40 USD
0.025 mg
12-452-C025
Delivery 1 week
73.70 USD
Variant
0.1 mg
12-452-C100
In stock
147.40 USD
0.025 mg
12-452-C025
Delivery 1 week
73.70 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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