Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD5 Purified

Anti-Hu CD5 Purified

Anti-Hu CD5 Purified
Regulatory status
RUO
Antigen
CD5
Clone
CRIS1
Format
Purified
Reactivity
Human
Application
ELISA, IP, WB, FC (QC tested), IHC(F)
Variant
0.1 mg
11-482-C100
In stock
130.00 USD
0.025 mg
11-482-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Workshop
HLDA I: WS Code T 29; HLDA III: WS Code T 530
Application
ELISA, IP, WB, FC (QC tested), IHC(F)
Application details
ELISA: The antibody CRIS1 can be used in the sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32
Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line.
Western blotting: Recommended dilution: 1-2 μg/ml; positive control: Jurkat human leukemia T-cell line, HPB human leukemia T-cell line.
Reactivity
Human
Immunogen
stimulated human leukocytes
Other names
T1, LEU1
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-482 FC
Flow cytometry analysis of human peripheral blood lymphocytes (PBL) stained with anti-human CD5 (CRIS1) antibody (blue; dilution of purified antibody 1 μg/ml); overlay with mouse IgG2a isotype control (red).

General references:

Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19(5):849-55.
PubMed
Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26(1-3):255-63.
PubMed

Product specific references:

Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996 Jan;5(1):167-9.
PubMed
Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987).
Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9.
PubMed
Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992 Mar 1;148(5):1287-93.
PubMed
Variant
0.1 mg
11-482-C100
In stock
130.00 USD
0.025 mg
11-482-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
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