Anti-Hu CD5 Purified

Anti-Hu CD5 Purified
Regulatory status
RUO
Antigen
CD5
Clone
CRIS1
Format
Purified
Reactivity
Human
Application
ELISA, IP, WB, FC (QC tested), IHC(F)
Variant
0.1 mg
11-482-C100
In stock

0.025 mg
11-482-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Workshop
HLDA I: WS Code T 29; HLDA III: WS Code T 530
Application
ELISA, IP, WB, FC (QC tested), IHC(F)
Reactivity
Human
Immunogen
stimulated human leukocytes
Other names
T1, LEU1
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-482 FC
Flow cytometry analysis of human peripheral blood lymphocytes (PBL) stained with CRIS1 antibody (blue; dilution of purified antibody 1 μg/ml); overlay with mouse IgG2a isotype control (red).

General references:

Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19(5):849-55.
PubMed
Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26(1-3):255-63.
PubMed

Product specific references:

Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996 Jan;5(1):167-9.
PubMed
Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987).
Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9.
PubMed
Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992 Mar 1;148(5):1287-93.
PubMed
Variant
0.1 mg
11-482-C100
In stock

0.025 mg
11-482-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock