Anti-Hu CD5 FITC

Anti-Hu CD5 FITC
Regulatory status
RUO
Antigen
CD5
Clone
CRIS1
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-482-T100
In stock
198.00 USD

25 tests
1F-482-T025
Delivery 1 week
99.00 USD
Variant
100 tests
1F-482-T100
In stock
198.00 USD

25 tests
1F-482-T025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Workshop
HLDA I: WS Code T 29; HLDA III: WS Code T 530
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
stimulated human leukocytes
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
T1, LEU1
Antigen description
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Entrez Gene ID 921
UniProt ID P06127
1F-482_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (CRIS1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
1F-482_FC_Histogram
Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (CRIS1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

General references:

Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19(5):849-55.
PubMed
Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26(1-3):255-63.
PubMed

Product specific references:

Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996 Jan;5(1):167-9.
PubMed
Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987).
Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9.
PubMed
Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992 Mar 1;148(5):1287-93.
PubMed
Variant
100 tests
1F-482-T100
In stock
198.00 USD

25 tests
1F-482-T025
Delivery 1 week
99.00 USD
Variant
100 tests
1F-482-T100
In stock
198.00 USD

25 tests
1F-482-T025
Delivery 1 week
99.00 USD

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