Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD49e Purified

Anti-Hu CD49e Purified

Regulatory status

RUO

Antigen

CD49e

Clone

SAM1

Format

Purified

Reactivity

Non-human primates, Human

Application

IP, FC (QC tested), IHC(F)

Variant

0.1 mg

11-914-C100

In stock

154.00 USD

Variant

0.1 mg

11-914-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody SAM1 recognizes an extracellular epitope of CD49e (integrin 5 alpha), a transmembrane glycoprotein expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells.

Application

IP, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Non-human primates, Human

Immunogen

U937 cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

VLA5 alpha, integrin 5 alpha, FNRA

Antigen description

CD49e (VLA5 alpha) is a type I transmembrane glycoprotein of the integrin alpha subclass (intergrin 5 alpha), expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells. It binds to RGD sequence in fibronectin and to neural adhesion molecule L1. CD49e interactions are important for maintaining the integrity of the endothelial monolayer, as well as it is involved in monocyte migration, T cell costimulation, regulation of cell survival, and other.

Entrez Gene ID 3678

UniProt ID P08648

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49e (SAM1) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Separation of human CD49e positive monocytes (red-filled) from human CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD49e (SAM1) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Product specific references:

Blase L, Merling A, Engelmann S, Möller P, Schwartz-Albiez R: Characterization of cell surface-expressed proteochondroitin sulfate of pre-B Nalm-6 cells and its possible role in laminin adhesion. Leukemia 1996 Jun;10(6):1000-11.
PubMed

Tachimoto H, Hudson SA, Bochner BS: Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation. J Allergy Clin Immunol. 2001 Feb;107(2):302-9
PubMed

Malara A, Gruppi C, Rebuzzini P, Visai L, Perotti C, Moratti R, Balduini C, Tira ME, Balduini A: Megakaryocyte-matrix interaction within bone marrow: new roles for fibronectin and factor XIII-A. Blood 2011 Feb 24;117(8):2476-83.
PubMed

Lessey BA, Castelbaum AJ, Buck CA, Lei Y, Yowell CW, Sun J: Further characterization of endometrial integrins during the menstrual cycle and in pregnancy. Fertil Steril. 1994 Sep;62(3):497-506.
PubMed