Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD49e PE

Anti-Hu CD49e PE

Regulatory status

RUO

Antigen

CD49e

Clone

SAM1

Format

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-914-T100

In stock

242.00 USD

Variant

100 tests

1P-914-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody SAM1 recognizes an extracellular epitope of CD49e (integrin 5 alpha), a transmembrane glycoprotein expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

U937 cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

VLA5 alpha, integrin 5 alpha, FNRA

Antigen description

CD49e (VLA5 alpha) is a type I transmembrane glycoprotein of the integrin alpha subclass (intergrin 5 alpha), expressed on thymocytes, early and activated B cells, monocytes, NK cells, dendritic cells, osteoblast and endothelial cells. It binds to RGD sequence in fibronectin and to neural adhesion molecule L1. CD49e interactions are important for maintaining the integrity of the endothelial monolayer, as well as it is involved in monocyte migration, T cell costimulation, regulation of cell survival, and other.

Entrez Gene ID 3678

UniProt ID P08648

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49e (SAM1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD49e positive monocytes (red-filled) from CD49e negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49e (SAM1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Blase L, Merling A, Engelmann S, Möller P, Schwartz-Albiez R: Characterization of cell surface-expressed proteochondroitin sulfate of pre-B Nalm-6 cells and its possible role in laminin adhesion. Leukemia 1996 Jun;10(6):1000-11.
PubMed

Tachimoto H, Hudson SA, Bochner BS: Acquisition and alteration of adhesion molecules during cultured human mast cell differentiation. J Allergy Clin Immunol. 2001 Feb;107(2):302-9
PubMed

Malara A, Gruppi C, Rebuzzini P, Visai L, Perotti C, Moratti R, Balduini C, Tira ME, Balduini A: Megakaryocyte-matrix interaction within bone marrow: new roles for fibronectin and factor XIII-A. Blood 2011 Feb 24;117(8):2476-83.
PubMed

Lessey BA, Castelbaum AJ, Buck CA, Lei Y, Yowell CW, Sun J: Further characterization of endometrial integrins during the menstrual cycle and in pregnancy. Fertil Steril. 1994 Sep;62(3):497-506.
PubMed