Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD49d APC-Cy™7

Anti-Hu CD49d APC-Cy™7

Regulatory status

RUO

Antigen

CD49d

Clone

9F10

Format

APC-Cy™7

Reactivity

Sheep, Cat, Horse, Dog, Cow, Non-human primates, Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

T4-171-T100

In stock

282.70 USD

Variant

100 tests

T4-171-T100

In stock

282.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.

Workshop

HLDA V: WS Code S215

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Sheep, Cat, Horse, Dog, Cow, Non-human primates, Human

Preparation

Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

ITGA4, VLA-4 alpha

Antigen description

CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.

Entrez Gene ID 3676

UniProt ID P13612

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49d (9F10) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD49d positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49d (9F10) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Stenner MP, Waschbisch A, Buck D, Doerck S, Einsele H, Toyka KV, Wiendl H: Effects of natalizumab treatment on Foxp3+ T regulatory cells. PLoS One. 2008 Oct 6;3(10):e3319.
PubMed

Chigaev A, Waller A, Amit O, Halip L, Bologa CG, Sklar LA: Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation. J Biol Chem. 2009 May 22;284(21):14337-46
PubMed

Schneider-Hohendorf T, Rossaint J, Mohan H, Böning D, Breuer J, Kuhlmann T, Gross CC, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H: VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. J Exp Med. 2014 Aug 25;211(9):1833-46.
PubMed

Yoon SO, Lee IY, Zhang X, Zapata MC, Choi YS: CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells. FEBS Open Bio. 2014 Apr 13;4:370-6.
PubMed

Zhang XF, Feng MF: Adherence of human monocytes and NK cells to human TNF-alpha-stimulated porcine endothelial cells. Immunol Cell Biol. 2000 Dec;78(6):633-40.
PubMed

Soler D, Chapman T, Yang LL, Wyant T, Egan R, Fedyk ER: The binding specificity and selective antagonism of vedolizumab, an anti-alpha4beta7 integrin therapeutic antibody in development for inflammatory bowel diseases. J Pharmacol Exp Ther. 2009 Sep;330(3):864-75.
PubMed