Anti-Hu CD49d APC-Cy™7

Anti-Hu CD49d APC-Cy™7
Regulatory status
RUO
Antigen
CD49d
Clone
9F10
Format
APC-Cy™7
Reactivity
Sheep, Cat, Horse, Dog, Cow, Non-human primates, Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
100 tests
T4-171-T100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.
Workshop
HLDA V: WS Code S215
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Sheep, Cat, Horse, Dog, Cow, Non-human primates, Human
Other names
ITGA4, Integrin alpha-4, VLA-4 alpha, VLA4 alpha
Preparation
The purified antibody is conjugated with tandem dye APC-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
Licence note
Cy™ and CyDye™ are registered trademarks of GE Healthcare.
T4-171_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49d (9F10) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T4-171_FC_Histogram
Separation of human CD49d positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49d (9F10) APC-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Stenner MP, Waschbisch A, Buck D, Doerck S, Einsele H, Toyka KV, Wiendl H: Effects of natalizumab treatment on Foxp3+ T regulatory cells. PLoS One. 2008 Oct 6;3(10):e3319.
PubMed
Chigaev A, Waller A, Amit O, Halip L, Bologa CG, Sklar LA: Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation. J Biol Chem. 2009 May 22;284(21):14337-46
PubMed
Schneider-Hohendorf T, Rossaint J, Mohan H, Böning D, Breuer J, Kuhlmann T, Gross CC, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H: VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. J Exp Med. 2014 Aug 25;211(9):1833-46.
PubMed
Yoon SO, Lee IY, Zhang X, Zapata MC, Choi YS: CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells. FEBS Open Bio. 2014 Apr 13;4:370-6.
PubMed
Zhang XF, Feng MF: Adherence of human monocytes and NK cells to human TNF-alpha-stimulated porcine endothelial cells. Immunol Cell Biol. 2000 Dec;78(6):633-40.
PubMed
Soler D, Chapman T, Yang LL, Wyant T, Egan R, Fedyk ER: The binding specificity and selective antagonism of vedolizumab, an anti-alpha4beta7 integrin therapeutic antibody in development for inflammatory bowel diseases. J Pharmacol Exp Ther. 2009 Sep;330(3):864-75.
PubMed
Variant
100 tests
T4-171-T100
In stock
Variant
0.1 mg
11-261-C100
In stock