Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD49c Purified

Anti-Hu CD49c Purified

Regulatory status

RUO

Antigen

CD49c

Clone

ASC-1

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-137-C100

In stock

143.00 USD

0.025 mg

11-137-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-137-C100

In stock

143.00 USD

0.025 mg

11-137-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody ASC-1 recognizes an extracellular epitope of CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.

Workshop

HLDA VI: WS Code A002

Application

IP, WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Human SSC-9 cell line (squamous cell carcinoma)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ITGA3, VLA-3 alpha, FRP-2, FRP2, GAPB3

Antigen description

CD49c / Integrin alpha 3 is a type I transmembrane glycoprotein proteolytically cleaved into two disulfide linked chains. It noncovalently associates with CD29 (integrin beta 1) to form the VLA-3 complex, an adhesion receptor for extracellular matrix components (fibronectin, laminin 1, laminin 5, entactin, and collagen). It is expressed on adherent cells, mainly on fibroblasts, epithelial cells and endothelial cells.

Entrez Gene ID 3675

UniProt ID P26006

Separation of HeLa cells stained using anti-human CD49c (ASC-1) purified antibody (concentration in sample 16 μg/ml, GAM APC, red) from HeLa cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).

Product specific references:

Skubitz AP, Bast RC, Wayner EA, Letourneau PC, Wilke MS: Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. Am J Pathol. 1996 May;148(5):1445-61.
PubMed

Pattaramalai S, Skubitz KM, Skubitz AP: A novel recognition site on laminin for the alpha 3 beta 1 integrin. Exp Cell Res. 1996 Feb 1;222(2):281-90.
PubMed