Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD49c APC

Anti-Hu CD49c APC

Regulatory status

RUO

Antigen

CD49c

Clone

ASC-1

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-137-T100

In stock

242.00 USD

Variant

100 tests

1A-137-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody ASC-1 recognizes an extracellular epitope of CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.

Workshop

HLDA VI: WS Code A002

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human SSC-9 cell line (squamous cell carcinoma)

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ITGA3, VLA-3 alpha, FRP-2, FRP2, GAPB3

Antigen description

CD49c / Integrin alpha 3 is a type I transmembrane glycoprotein proteolytically cleaved into two disulfide linked chains. It noncovalently associates with CD29 (integrin beta 1) to form the VLA-3 complex, an adhesion receptor for extracellular matrix components (fibronectin, laminin 1, laminin 5, entactin, and collagen). It is expressed on adherent cells, mainly on fibroblasts, epithelial cells and endothelial cells.

Entrez Gene ID 3675

UniProt ID P26006

Separation of HeLa cells stained using anti-human CD49c (ASC-1) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1.67 μg/ml, same as CD49c APC concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Skubitz AP, Bast RC, Wayner EA, Letourneau PC, Wilke MS: Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. Am J Pathol. 1996 May;148(5):1445-61.
PubMed

Pattaramalai S, Skubitz KM, Skubitz AP: A novel recognition site on laminin for the alpha 3 beta 1 integrin. Exp Cell Res. 1996 Feb 1;222(2):281-90.
PubMed