Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD49b PE

Anti-Hu CD49b PE

Regulatory status

RUO

Antigen

CD49b

Clone

AK7

Format

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-151-T100

In stock

242.00 USD

25 tests

1P-151-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-151-T100

In stock

242.00 USD

25 tests

1P-151-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody AK7 recognizes an extracellular epitope of CD49b, a 160-165 kDa alpha subunit of VLA-2 integrin complex expressed on platelets, megakaryocytes, activated T and B cells, monocytes, epithelial cells, endothelial cells and fibroblasts.

Workshop

HLDA V: WS Code S190

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

Human platelets

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ITGA2, VLA-2 alpha, GPIa

Antigen description

CD49b the integrin alpha 2 chain, associates with CD29 (integrin beta 1 chain) to form VLA-2 integrin complex, which plays a critical role in the processes of lymphocyte adhesion and activation. VLA-2 serves as a receptor for collagen, laminin, and fibronectin and also regulates the extracellular matrix synthesis and organization. CD49b has been used to identify NK cells, and coexpressed with CD223 (LAG-3) it identifies CD4+ T regulatory type 1 cells (Tr1).

Entrez Gene ID 3673

UniProt ID P17301

Flow cytometry analysis (surface staining) of CD49b in human peripheral blood with anti-CD49b (AK7) PE.

Product specific references:

Müller K, Schinn M, Reichrath J, Meineke V: 1alpha,25-Dihydroxyvitamin D3 modulates the response of human keratinocytes to ionizing radiation exposure. Anticancer Res. 2006 Jul-Aug;26(4A):2735-41.
PubMed

Engl T, Makarević J, Relja B, Natsheh I, Müller I, Beecken WD, Jonas D, Blaheta RA: Mycophenolate mofetil modulates adhesion receptors of the beta1 integrin family on tumor cells: impact on tumor recurrence and malignancy. BMC Cancer. 2005 Jan 11;5:4.
PubMed

Sutavani RV, Bradley RG, Ramage JM, Jackson AM, Durrant LG, Spendlove I: CD55 costimulation induces differentiation of a discrete T regulatory type 1 cell population with a stable phenotype. J Immunol. 2013 Dec 15;191(12):5895-903.
PubMed

Gamble JR, Matthias LJ, Meyer G, Kaur P, Russ G, Faull R, Berndt MC, Vadas MA: Regulation of in vitro capillary tube formation by anti-integrin antibodies. J Cell Biol. 1993 May;121(4):931-43.
PubMed

Londrigan SL, Hewish MJ, Thomson MJ, Sanders GM, Mustafa H, Coulson BS: Growth of rotaviruses in continuous human and monkey cell lines that vary in their expression of integrins. J Gen Virol. 2000 Sep;81(Pt 9):2203-13.
PubMed

Werr J, Johansson J, Eriksson EE, Hedqvist P, Ruoslahti E, Lindbom L: Integrin alpha(2)beta(1) (VLA-2) is a principal receptor used by neutrophils for locomotion in extravascular tissue. Blood. 2000 Mar 1;95(5):1804-9.
PubMed

Ammon C, Meyer SP, Schwarzfischer L, Krause SW, Andreesen R, Kreutz M: Comparative analysis of integrin expression on monocyte-derived macrophages and monocyte-derived dendritic cells. Immunology. 2000 Jul;100(3):364-9.
PubMed

Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK: VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment. J Virol. 2002 Feb;76(3):1109-23.
PubMed

Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P: A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004 Jul 19;200(2):123-35.
PubMed