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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD49a PE
Anti-Hu CD49a PE
Regulatory status
RUO
Antigen
CD49a
Clone
TS2/7
Format
PE
Reactivity
Human, Non-human primates
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-873-T100
In stock
242.00 USD
Variant
100 tests
1P-873-T100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody TS2/7 recognizes an extracellular epitope of CD49a, the alpha 1 chain of VLA integrin complex, that serves as an adhesion receptor for collagen IV and laminin 1.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Immunogen
human CTL line
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
alpha 1 integrin, VLA-1 alpha, ITGA1
Antigen description
CD49a is the alpha 1 chain of VLA integrin complex (together with CD29, serving as the beta 1 chain), and is expressed on activated T cells, monocytes, NK cells, cultured neuronal cells, melanoma cells, mesenchymal cells (including smooth muscle cells), fibroblasts, hepatocytes, and microvascular endothelium. It binds to collagen IV and laminin 1. It is important for leukocyte migration into tissues. It is upregulated in inflammatory tissues, such as inflammed intestine.
Entrez Gene ID
3672
UniProt ID
P56199
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD49a positive lymphocytes (red-filled) from CD49a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of HeLa cells stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/ml, same as CD49a PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.
Product specific references:
Melssen MM, Olson W, Wages NA, Capaldo BJ, Mauldin IS, Mahmutovic A, Hutchison C, Melief CJ, Bullock TN, Engelhard VH, Slingluff CL: Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma. Oncoimmunology. 2018 Aug 6;7(10):e1490855.
PubMed
Luque A, Gómez M, Puzon W, Takada Y, Sánchez-Madrid F, Cabañas C: Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common beta 1 chain. J Biol Chem. 1996 May 10;271(19):11067-75.
PubMed
Canfield SM, Khakoo AY: The nonintegrin laminin binding protein (p67 LBP) is expressed on a subset of activated human T lymphocytes and, together with the integrin very late activation antigen-6, mediates avid cellular adherence to laminin. J Immunol. 1999 Sep 15;163(6):3430-40.
PubMed
Martrus G, Kautz T, Lunemann S, Richert L, Glau L, Salzberger W, Goebels H, Langeneckert A, Hess L, Poch T, Schramm C, Oldhafer KJ, Koch M, Tolosa E, Nashan B, Altfeld M: Proliferative capacity exhibited by human liver-resident CD49a+CD25+ NK cells. PLoS One. 2017 Aug 9;12(8):e0182532.
PubMed
Thomson-Luque R, Wang C, Tumgia FB, Xu S, Szekeres K, Conway A, Adapa SR, Barnes SJ, Adams JH, Jiang RH: In-depth phenotypic characterization of reticulocyte maturation using mass cytometry. Blood Cells Mol Dis. 2018 Sep;72:22-33.
PubMed
Marquardt N, Kekäläinen E, Chen P, Lourda M, Wilson JN, Scharenberg M, Bergman P, Al-Ameri M, Hård J, Mold JE, Ljunggren HG, Michaëlsson J. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells. Nat Commun. 2019 Aug 26;10(1):3841.
PubMed
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
1P-873-T100
In stock
242.00 USD
Variant
100 tests
1P-873-T100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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