Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD49a PE

Anti-Hu CD49a PE

Regulatory status

RUO

Antigen

CD49a

Clone

TS2/7

Format

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-873-T100

In stock

242.00 USD

Variant

100 tests

1P-873-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody TS2/7 recognizes an extracellular epitope of CD49a, the alpha 1 chain of VLA integrin complex, that serves as an adhesion receptor for collagen IV and laminin 1.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

human CTL line

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

alpha 1 integrin, VLA-1 alpha, ITGA1

Antigen description

CD49a is the alpha 1 chain of VLA integrin complex (together with CD29, serving as the beta 1 chain), and is expressed on activated T cells, monocytes, NK cells, cultured neuronal cells, melanoma cells, mesenchymal cells (including smooth muscle cells), fibroblasts, hepatocytes, and microvascular endothelium. It binds to collagen IV and laminin 1. It is important for leukocyte migration into tissues. It is upregulated in inflammatory tissues, such as inflammed intestine.

Entrez Gene ID 3672

UniProt ID P56199

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD49a positive lymphocytes (red-filled) from CD49a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of HeLa cells stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/ml, same as CD49a PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.

Product specific references:

Melssen MM, Olson W, Wages NA, Capaldo BJ, Mauldin IS, Mahmutovic A, Hutchison C, Melief CJ, Bullock TN, Engelhard VH, Slingluff CL: Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma. Oncoimmunology. 2018 Aug 6;7(10):e1490855.
PubMed

Luque A, Gómez M, Puzon W, Takada Y, Sánchez-Madrid F, Cabañas C: Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common beta 1 chain. J Biol Chem. 1996 May 10;271(19):11067-75.
PubMed

Canfield SM, Khakoo AY: The nonintegrin laminin binding protein (p67 LBP) is expressed on a subset of activated human T lymphocytes and, together with the integrin very late activation antigen-6, mediates avid cellular adherence to laminin. J Immunol. 1999 Sep 15;163(6):3430-40.
PubMed

Martrus G, Kautz T, Lunemann S, Richert L, Glau L, Salzberger W, Goebels H, Langeneckert A, Hess L, Poch T, Schramm C, Oldhafer KJ, Koch M, Tolosa E, Nashan B, Altfeld M: Proliferative capacity exhibited by human liver-resident CD49a+CD25+ NK cells. PLoS One. 2017 Aug 9;12(8):e0182532.
PubMed

Thomson-Luque R, Wang C, Tumgia FB, Xu S, Szekeres K, Conway A, Adapa SR, Barnes SJ, Adams JH, Jiang RH: In-depth phenotypic characterization of reticulocyte maturation using mass cytometry. Blood Cells Mol Dis. 2018 Sep;72:22-33.
PubMed

Marquardt N, Kekäläinen E, Chen P, Lourda M, Wilson JN, Scharenberg M, Bergman P, Al-Ameri M, Hård J, Mold JE, Ljunggren HG, Michaëlsson J. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells. Nat Commun. 2019 Aug 26;10(1):3841.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed