Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD45RB Purified

Anti-Hu CD45RB Purified

Regulatory status

RUO

Antigen

CD45RB

Clone

MEM-55

Format

Purified

Reactivity

Non-human primates, Human

Application

IP, WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-224-C100

In stock

143.00 USD

0.025 mg

11-224-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-224-C100

In stock

143.00 USD

0.025 mg

11-224-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-55 recognizes a siliadase-sensitive extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.

Workshop

HLDA III: WS Code NL 358; HLDA IV: WS Code NL 2; HLDA V: WS Code T T-151; HLDA V: WS Code T T-CD45.08

Application

IP, WB, FC (QC tested), IHC(P)

Application details

Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Flow cytometry: Recommended dilution: 0.5-2 μg/ml.

Reactivity

Non-human primates, Human

Immunogen

Human thymocytes and T lymphocytes.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PTPRCR, T200RB

Antigen description

CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.

Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-CD45RB (MEM-55).

Separation of human CD45RB positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD45RB (MEM-55) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD45RB (MEM-55) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-CD45RB (MEM-55). Commercially tested by LifeSpan BioSciences.

General references:

Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice. Neurosci Lett. 2004 May 13;362(1):26-30.
PubMed

Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302.
PubMed

Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65.
PubMed

Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25.
PubMed

Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90.
PubMed

Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19
PubMed

Product specific references:

Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90(1):5-19.
PubMed

Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34.
PubMed

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).