Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD45RA Purified

Anti-Hu CD45RA Purified

Regulatory status

RUO

Antigen

CD45RA

Clone

MEM-56

Format

Purified

Reactivity

Human

Application

IP, MC (CyTOF), WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-223-C100

In stock

143.00 USD

0.025 mg

11-223-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-223-C100

In stock

143.00 USD

0.025 mg

11-223-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The antibody MEM-56 reacts with an extracellular epitope of CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes.

Workshop

HLDA IV: WS Code NL 907

Application

IP, MC (CyTOF), WB, FC (QC tested), IHC(P)

Application details

Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Flow cytometry: Recommended dilution: 0.3-4 μg/ml.

Reactivity

Human

Immunogen

Human thymocytes and T lymphocytes.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PTPRCR, T200RA

Antigen description

CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RA is expressed e.g. on naïve T cells and normal plasma cells.

Western blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD45 control antibody Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions

Separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 μg/ml, GAM APC).

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD45RA (MEM-56), 5 μg/ml. Commercially tested by LifeSpan BioSciences.

Mass cytometry (surface staining) of human peripheral blood with anti-CD45RA (MEM-56) 163Dy. Gated on singlets.

General references:

Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302.
PubMed

Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65.
PubMed

Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25.
PubMed

Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90.
PubMed

Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19
PubMed

Product specific references:

Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha). 1989;35(5):289-97.
PubMed

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).