Anti-Hu CD45 Purified

Anti-Hu CD45 Purified
Regulatory status
RUO
Antigen
CD45
Clone
MEM-28
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-222-C100
In stock
130.00 USD

1.0 mg
11-222-M001
In stock
390.00 USD

0.025 mg
11-222-C025
In stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
390.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Workshop
HLDA III: WS Code NL 833a
Application details
Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed.
Immunocytochemistry: Recommended dilution: 10 μg/ml; paraformaldehyde fixation can be used.
Western blotting: Recommended dilution: 1 μg/ml; non-reducing conditions, band around 200-250 kDa; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel.
Flow cytometry: Recommended dilution: 1 μg/ml.
Reactivity
Human
Negative species
Horse
Immunogen
Human thymocytes and T lymphocytes.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LCA, T200, LY5, B220, GP180, TPC
Antigen description
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Entrez Gene ID 5788
UniProt ID P08575
11-222_ICC
Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28, green). DNA visualized by DAPI (blue)
11-222_IHC
Immunohistochemistry staining of human small intestine (paraffin-embedded sections) with anti-CD45 (MEM-28), 10 μg/ml. Commercially tested by LifeSpan BioSciences.
11-222 FC
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (MEM-28) purified, GAM-APC.

General references:

Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302.
PubMed
Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65.
PubMed
Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25.
PubMed
Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90.
PubMed
Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19.
PubMed

Product specific references:

Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).
Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34.
PubMed
Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8.
PubMed
Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
PubMed
Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90(1):5-19.
PubMed
Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed
McCormack E, Mujić M, Osdal T, Bruserud Ø, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555.
PubMed
Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95.
PubMed
Variant
0.1 mg
11-222-C100
In stock
130.00 USD

1.0 mg
11-222-M001
In stock
390.00 USD

0.025 mg
11-222-C025
In stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
390.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD

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