Clinical products
Premium antibodies (GMP)
Intracellular staining
Surface staining
Hematology
Kits
Lysing solutions
Multicolor cocktails
Single color antibodies
Immunodeficiency
Kits
Lysing solutions
Mitogens
Multicolor cocktails
Single color antibodies
Reproductive immunology
Kits
Lysing solutions
Multicolor cocktails
New products
Allergy
Kits
Recombinant allergens
Allergen extracts
Other allergens
Research products
Antibodies
CD and related antigens
Blood group antigens
Multicolor cocktails
Serum, plasma, and secreted proteins
Cytoskeleton and ECM
Isotype controls
MHC antigens
Nucleus
Other intracellular antigens
Small organic molecules
Tags and labels
Microbiology and virology
Accessory reagents
Annexin V
DNA-binding probes
Lineage cocktail
Lysing solutions
Recombinant proteins
Streptavidin
Recombinant allergens
Animal
Food
House Dust Mite
Mold
Pollen
Venom
Kits
New products
Dry Reagents
About exbio
Company
OEM and Commercial Supplies
Events and Conferences
Blog
Inquiries
Technical resources
International distributors
Contact
Jobs
IVDR
E-mail
*
Password
*
Remember me
Forgotten password
Log in
Register
E-mail
Send request
Log in
Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD45 Purified
Anti-Hu CD45 Purified
Regulatory status
RUO
Antigen
CD45
Clone
HI30
Format
Purified
Reactivity
Human, Non-human primates
Application
IP, WB, FC (QC tested), IHC-F, IHC-P, ICC
Variant
0.1 mg
11-684-C100
In stock
143.00 USD
0.025 mg
11-684-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-684-C100
In stock
143.00 USD
0.025 mg
11-684-C025
Delivery 1 week
71.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Workshop
HLDA IV: WS Code N816
Application
IP, WB, FC (QC tested), IHC-F, IHC-P, ICC
Application details
Western blotting: non-reducing conditions; recommended dilution: 1 μg/ml; 6-7% gel recommended (band around 180-250 kDa).
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human, Non-human primates
Immunogen
human peripheral blood leukocytes
Concentration
1 mg/ml
Preparation
Purified by protein-G affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LCA, T200, LY5, B220, GP180, TPC
Antigen description
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Entrez Gene ID
5788
UniProt ID
P08575
Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD45 (HI30) purified, GAM-APC.
Anti-Hu CD45 Purified (clone HI30) works in WB application under non-reducing conditions.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% SDS-PAGE gel.
Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody HI30 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Multiplex fluorescent Western blot detection was performed.
CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.
General references:
Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302.
PubMed
Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65.
PubMed
Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25.
PubMed
Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90.
PubMed
Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19.
PubMed
Product specific references:
Tembhare PR, Subramanian PG, Sehgal K, Yajamanam B, Kumar A, Gadge V, Inamdar N, Gujral S: Immunophenotypic profile of plasma cell leukemia: a retrospective study in a reference cancer center in India and review of literature. Indian J Pathol Microbiol. 2011 Apr-Jun;54(2):294-8.
PubMed
Cubillos-Ruiz JR, Martinez D, Scarlett UK, Rutkowski MR, Nesbeth YC, Camposeco-Jacobs AL, Conejo-Garcia JR: CD277 is a negative co-stimulatory molecule universally expressed by ovarian cancer microenvironmental cells. Oncotarget. 2010 Sep;1(5):329-38.
PubMed
Song L, Ara T, Wu HW, Woo CW, Reynolds CP, Seeger RC, DeClerck YA, Thiele CJ, Sposto R, Metelitsa LS: Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma. J Clin Invest. 2007 Sep;117(9):2702-12.
PubMed
Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F: Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005 Jun;26(12):1196-204.
PubMed
Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW Jr, Arthur LO, Ott DE, Lifson JD: Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation. J Virol. 2001 Jul;75(13):6173-82.
PubMed
Yamada T, Zhu D, Saxon A, Zhang K: CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphatase. J Biol Chem. 2002 Aug 9;277(32):28830-5.
PubMed
Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE: A novel marker of human endometrial mesenchymal stem-like cells. Cell Transplant. 2012;21(10):2201-14.
PubMed
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-684-C100
In stock
143.00 USD
0.025 mg
11-684-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-684-C100
In stock
143.00 USD
0.025 mg
11-684-C025
Delivery 1 week
71.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
Related products
ED7066
EXCELLYSE Easy
-134%
Clone
Reg. status
CE IVD
Application
FC
100 ml
220.00 USD
ED7065
EXCELLYSE I
-134%
Clone
Reg. status
CE IVD
Application
FC
100 ml
66.00 USD
ED7068
EXCELLYSE Live
-134%
Clone
Reg. status
RUO
Application
FC
100 ml
55.00 USD
11-457-C100
Mouse IgG1 Isotype Control Purified
-134%
Clone
PPV-06
Reg. status
RUO
Application
ELISA, FC, FC-IC, IP, WB, IHC-F, IHC-P, ICC
0.1 mg
115.50 USD
11-632-C100
Mouse IgG1 Isotype Control Purified
-134%
Clone
MOPC-21
Reg. status
RUO
Application
ELISA, FC, FC-IC, IP, WB, IHC-F, IHC-P, ICC
0.1 mg
115.50 USD
Continue to checkout
×
Zavřít