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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD44 Purified
Anti-Hu CD44 Purified
Regulatory status
RUO
Antigen
CD44
Clone
MEM-263
Format
Purified
Reactivity
Pig, Human, Dog
Application
IP, WB, FC (QC tested), IHC-P
Variant
0.1 mg
11-341-C100
In stock
154.00 USD
0.025 mg
11-341-C025
In stock
77.00 USD
Variant
0.1 mg
11-341-C100
In stock
154.00 USD
0.025 mg
11-341-C025
In stock
77.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
Workshop
HLDA III: WS Code T 155
Application
IP, WB, FC (QC tested), IHC-P
Application details
Flow cytometry: Recommended dilution: 4 μg/ml.
Western blotting: Recommended dilution: 2 μg/ml, 60 min on vertical incubator; positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6% separating gel.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: uterus, myometrium.
Reactivity
Pig, Human, Dog
Immunogen
COS-7 cells (African Green Monkey).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR
Antigen description
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
Entrez Gene ID
960
UniProt ID
P16070
Western blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL
Separation of human CD44 positive lymphocytes (red-filled) from CD44 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD44 (MEM-263) purified antibody (concentration in sample 4 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD44 (MEM-263) purified antibody (concentration in sample 4 μg/ml) GAM APC.
Western blotting analysis of CD44 in MOLT-4 cells (lane 1) and HeLa cells (lane 2) using anti-CD44 (MEM-263) purified; non-reducing conditions.
General references:
Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M, Hascall VC, De Luca G, Passi A: Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging. J Biol Chem. 2007 Dec 12
PubMed
Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am J Respir Cell Mol Biol. 2007 Aug;37(2):248-53.
PubMed
Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G. Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA. Cell Mol Immunol. 2007 Feb;4(1):59-63.
PubMed
Subramaniam V, Gardner H, Jothy S: Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells. Exp Mol Pathol. 2007 Dec;83(3):341-6.
PubMed
Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S: CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation. Exp Mol Pathol. 2007 Oct;83(2):207-15.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-341-C100
In stock
154.00 USD
0.025 mg
11-341-C025
In stock
77.00 USD
Variant
0.1 mg
11-341-C100
In stock
154.00 USD
0.025 mg
11-341-C025
In stock
77.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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