Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD43 Purified

Anti-Hu CD43 Purified

Regulatory status

RUO

Antigen

CD43

Clone

MEM-59

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-220-C100

In stock

0.025 mg

11-220-C025

Out of stock

Variant

0.1 mg

11-261-C100

In stock

0.1 mg

11-261-C100

In stock

Product details

Images

References

Isotype

Mouse IgG1

Specificity

The antibody MEM-59 recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.

Workshop

HLDA IV: WS Code NL 604; HLDA V: WS Code AS S290

Application

IP, WB, FC (QC tested), IHC(P)

Reactivity

Human

Immunogen

Human T lymphocytes.

Other names

Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP

Preparation

Purified by protein-A affinity chromatography

Formulation

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD43 (MEM-59). Commercially tested by LifeSpan BioSciences.

Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL

General references:

Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. Oncogene. 2004 Apr 1;23(14):2523-30.
PubMed

Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, Anděra L, Hořejší V, Stockinger H: LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Mol Immunol. 2007 Nov 8
PubMed

Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T: CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Oncogene. 2007 Sep 24
PubMed

Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T-cell trafficking. Blood. 2007 Oct 15;110(8):2974-82.
PubMed

Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 nFeb;26(2):153-61.
PubMed

Product specific references:

Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed

Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
PubMed

Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed

Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
PubMed

Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol. 1995 Apr;25(4):1051-5.
PubMed

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha). 1988;34(4):255-65.
PubMed

Variant

0.1 mg

11-220-C100

In stock

0.025 mg

11-220-C025

Out of stock

Variant

0.1 mg

11-261-C100

In stock

0.1 mg

11-261-C100

In stock

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