Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD43 Purified

Anti-Hu CD43 Purified

Anti-Hu CD43 Purified
Regulatory status
RUO
Antigen
CD43
Clone
MEM-59
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested), IHC(P)
Variant
0.1 mg
11-220-C100
In stock
0.025 mg
11-220-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock
0.1 mg
11-261-C100
In stock
Contact us
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-59 recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
Workshop
HLDA IV: WS Code NL 604; HLDA V: WS Code AS S290
Application
IP, WB, FC (QC tested), IHC(P)
Reactivity
Human
Immunogen
Human T lymphocytes.
Other names
Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-220 IHC
Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD43 (MEM-59). Commercially tested by LifeSpan BioSciences.
11-220 WB
Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-human CD43 (MEM-59). Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL

General references:

Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. Oncogene. 2004 Apr 1;23(14):2523-30.
PubMed
Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, Anděra L, Hořejší V, Stockinger H: LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Mol Immunol. 2007 Nov 8
PubMed
Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T: CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Oncogene. 2007 Sep 24
PubMed
Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T-cell trafficking. Blood. 2007 Oct 15;110(8):2974-82.
PubMed
Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 nFeb;26(2):153-61.
PubMed

Product specific references:

Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed
Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
PubMed
Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed
Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
PubMed
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol. 1995 Apr;25(4):1051-5.
PubMed
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha). 1988;34(4):255-65.
PubMed
Variant
0.1 mg
11-220-C100
In stock
0.025 mg
11-220-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock
0.1 mg
11-261-C100
In stock
Contact us
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