Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD42b Purified

Anti-Hu CD42b Purified

Regulatory status

RUO

Antigen

CD42b

Clone

HIP1

Format

Purified

Reactivity

Non-human primates, Human

Application

FC (QC tested), IHC(F)

Variant

0.1 mg

11-367-C100

In stock

154.00 USD

0.025 mg

11-367-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-367-C100

In stock

154.00 USD

0.025 mg

11-367-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody HIP1 reacts with an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.

Workshop

HLDA IV: WS Code P 40

Application

FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Non-human primates, Human

Immunogen

Peripheral blood mononuclear cells of a patient suffering with CLL.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3

Antigen description

CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.

Entrez Gene ID 2811

UniProt ID P07359

Flow cytometry surface staining pattern of human peripheral blood cells stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 μg/ml) GAM APC.

General references:

Bergmeier W, Piffath CL, Goerge T, Cifuni SM, Ruggeri ZM, Ware J, Wagner DD: The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16900-5.
PubMed

Vanhoorelbeke K, Ulrichts H, Van de Walle G, Fontayne A, Deckmyn H: Inhibition of platelet glycoprotein Ib and its antithrombotic potential. Curr Pharm Des. 2007;13(26):2684-97.
PubMed

Jain S, Zuka M, Liu J, Russell S, Dent J, Guerrero JA, Forsyth J, Maruszak B, Gartner TK, Felding-Habermann B, Ware J. Platelet glycoprotein Ib alpha supports experimental lung metastasis. Proc Natl Acad Sci U S A. 2007 May 22;104(21):9024-8.
PubMed

Product specific references:

Leukocyte Typing IV., Knapp W et al. (Eds.), Oxford University Press (1989).

Takahashi R, Sekine N, Nakatake T: Influence of monoclonal antiplatelet glycoprotein antibodies on in vitro human megakaryocyte colony formation and proplatelet formation. Blood. 1999 Mar 15;93(6):1951-8.
PubMed