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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD42b Purified
Anti-Hu CD42b Purified
Regulatory status
RUO
Antigen
CD42b
Clone
AK2
Format
Purified
Reactivity
Human
Application
ELISA, IP, WB, FC (QC tested), ICC
Variant
0.1 mg
11-150-C100
In stock
143.00 USD
0.025 mg
11-150-C025
In stock
71.50 USD
Variant
0.1 mg
11-150-C100
In stock
143.00 USD
0.025 mg
11-150-C025
In stock
71.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Workshop
HLDA V: WS Code P024
Application
ELISA, IP, WB, FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
Human platelets
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3
Antigen description
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Entrez Gene ID
2811
UniProt ID
P07359
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD42b (AK2) purified antibody (concentration in sample 4 μg/ml) GAM APC.
Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42b (AK2) purified antibody (concentration in sample 4 μg/ml) GAM APC.
General references:
Bergmeier W, Piffath CL, Goerge T, Cifuni SM, Ruggeri ZM, Ware J, Wagner DD: The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16900-5.
PubMed
Vanhoorelbeke K, Ulrichts H, Van de Walle G, Fontayne A, Deckmyn H: Inhibition of platelet glycoprotein Ib and its antithrombotic potential. Curr Pharm Des. 2007;13(26):2684-97.
PubMed
Jain S, Zuka M, Liu J, Russell S, Dent J, Guerrero JA, Forsyth J, Maruszak B, Gartner TK, Felding-Habermann B, Ware J. Platelet glycoprotein Ib alpha supports experimental lung metastasis. Proc Natl Acad Sci U S A. 2007 May 22;104(21):9024-8.
PubMed
Product specific references:
Vettore S, Scandellari R, Moro S, Lombardi AM, Scapin M, Randi ML, Fabris F: Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant. Haematologica. 2008 Nov;93(11):1743-7. doi: 10.3324/haematol.12830.
PubMed
Welsh JD, Colace TV, Muthard RW, Stalker TJ, Brass LF, Diamond SL: Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse. J Thromb Haemost. 2012 Nov;10(11):2344-53.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-150-C100
In stock
143.00 USD
0.025 mg
11-150-C025
In stock
71.50 USD
Variant
0.1 mg
11-150-C100
In stock
143.00 USD
0.025 mg
11-150-C025
In stock
71.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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