Anti-Hu CD42b APC

Anti-Hu CD42b APC
Regulatory status
RUO
Antigen
CD42b
Clone
AK2
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-150-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Workshop
HLDA V: WS Code P024
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human platelets
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3
Antigen description
CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
Entrez Gene ID 2811
UniProt ID P07359
1A-150_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD42b (AK2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-150_FC_Histogram
Separation of human CD45 negative CD42b positive thrombocytes (red-filled) from CD42b negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42b (AK2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

General references:

Bergmeier W, Piffath CL, Goerge T, Cifuni SM, Ruggeri ZM, Ware J, Wagner DD: The role of platelet adhesion receptor GPIbalpha far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16900-5.
PubMed
Vanhoorelbeke K, Ulrichts H, Van de Walle G, Fontayne A, Deckmyn H: Inhibition of platelet glycoprotein Ib and its antithrombotic potential. Curr Pharm Des. 2007;13(26):2684-97.
PubMed
Jain S, Zuka M, Liu J, Russell S, Dent J, Guerrero JA, Forsyth J, Maruszak B, Gartner TK, Felding-Habermann B, Ware J. Platelet glycoprotein Ib alpha supports experimental lung metastasis. Proc Natl Acad Sci U S A. 2007 May 22;104(21):9024-8.
PubMed

Product specific references:

Vettore S, Scandellari R, Moro S, Lombardi AM, Scapin M, Randi ML, Fabris F: Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant. Haematologica. 2008 Nov;93(11):1743-7. doi: 10.3324/haematol.12830.
PubMed
Welsh JD, Colace TV, Muthard RW, Stalker TJ, Brass LF, Diamond SL: Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse. J Thromb Haemost. 2012 Nov;10(11):2344-53.
PubMed
Variant
100 tests
1A-150-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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