Anti-Hu CD42a APC

Anti-Hu CD42a APC
Regulatory status
RUO
Antigen
CD42a
Clone
GR-P
Format
APC
Reactivity
Dog, Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-732-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody GR-P (also known as GRP-P) recognizes an extracellular epitope of CD42a (glycoprotein 9), a 22 kDa transmembrane protein constitutively expressed on megakaryocytes and platelets.
Workshop
HLDA IV: WS Code P 35
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Dog, Human
Immunogen
Human acute lymphoblastic leukemia cells
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
GPIX, GP9
Antigen description
CD42a, also known as glycoprotein 9 (GPIX), composes together with GPIb alpha, GPIb beta and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. Defects in the gene encoding CD42a are a cause of Bernard-Soulier syndrome, also known as giant platelet disease. These patients have unusually large platelets and have a clinical bleeding tendency.
Entrez Gene ID 2815
UniProt ID P14770
1A-732_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD42a (GR-P) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-732_FC_Histogram
Separation of human thrombocytes (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD42a (GR-P) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Brown SB, Clarke MC, Magowan L, Sanderson H, Savill J: Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. J Biol Chem. 2000 Feb 25;275(8):5987-96.
PubMed
Vettore S, Scandellari R, Moro S, Lombardi AM, Scapin M, Randi ML, Fabris F: Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant. Haematologica. 2008 Nov;93(11):1743-7.
PubMed
Din JN, Aftab SM, Jubb AW, Carnegy FH, Lyall K, Sarma J, Newby DE, Flapan AD: Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans. Eur J Clin Nutr. 2011 Feb;65(2):234-9.
PubMed
Harding SA, Din JN, Sarma J, Josephs DH, Fox KA, Newby DE: Promotion of proinflammatory interactions between platelets and monocytes by unfractionated heparin. Heart. 2006 Nov;92(11):1635-8.
PubMed
Variant
100 tests
1A-732-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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