Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD41a Purified

Anti-Hu CD41a Purified

Regulatory status

RUO

Antigen

CD41a

Clone

HIP8

Format

Purified

Reactivity

Non-human primates, Human

Application

FC (QC tested), IHC(F)

Variant

0.1 mg

11-417-C100

In stock

154.00 USD

0.025 mg

11-417-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-417-C100

In stock

154.00 USD

0.025 mg

11-417-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody HIP8 reacts with an extracellular epitope on alpha (a) subunit of CD41 (heavy chain; 120 kDa). CD41 is mainly expressed on platelets and megakaryocytes. The antibody HIP8 blocks platelet aggregation and completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. HIP8 is useful in the morphological and physiological studies o platelets and megakaryocytes.

Workshop

HLDA IV: WS Code P 38

Application

FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Non-human primates, Human

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GT, GTA, GP2B, HPA3, GPIIb

Antigen description

CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.

Entrez Gene ID 3674

UniProt ID P08514

Flow cytometry (surface staining) of human peripheral blood cells with anti-CD41a (HIP8) purified, GAM-APC.

General references:

Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W: Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development. 2002 Apr;129(8):2003-13.
PubMed

Ferkowicz MJ, Starr M, Xie X, Li W, Johnson SA, Shelley WC, Morrison PR, Yoder MC: CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003 Sep;130(18):4393-403.
PubMed

Zhang J, Varas F, Stadtfeld M, Heck S, Faust N, Graf T: CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation. Exp Hematol. 2007 Mar;35(3):490-499.
PubMed

Debili N, Robin C, Schiavon V, Letestu R, Pflumio F, Mitjavila-Garcia MT, Coulombel L, Vainchenker W: Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates. Blood. 2001 Apr 1;97(7):2023-30.
PubMed

Product specific references:

Wu XW., Chen Z., Bao CX., et al., 1989: Production and application of anti-human platelet GPIIb/IIIa monoclonal antibodies. National Medical J. of China. 69(8):427
PubMed