Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD41a Pacific Blue™

Anti-Hu CD41a Pacific Blue™

Anti-Hu CD41a Pacific Blue™
Regulatory status
RUO
Antigen
CD41a
Clone
HIP8
Format
Pacific Blue™
Reactivity
Non-human primates, Human
Application
FC (QC tested)
Excitation laser
violet (405 nm)
Variant
100 tests
PB-417-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody HIP8 reacts with an extracellular epitope on alpha (a) subunit of CD41 (heavy chain; 120 kDa). CD41 is mainly expressed on platelets and megakaryocytes. The antibody HIP8 blocks platelet aggregation and completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. HIP8 is useful in the morphological and physiological studies o platelets and megakaryocytes.
Workshop
HLDA IV: WS Code P 38
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Other names
GT, GTA, GP2B, HPA3, GPIIb
Preparation
The purified antibody is conjugated with Pacific Blue™ under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
PB-417_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD41a (HIP8) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
PB-417_FC_Histogram
Separation of human CD41a positive thrombocytes (red-filled) from CD41a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD41a (HIP8) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W: Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development. 2002 Apr;129(8):2003-13.
PubMed
Ferkowicz MJ, Starr M, Xie X, Li W, Johnson SA, Shelley WC, Morrison PR, Yoder MC: CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003 Sep;130(18):4393-403.
PubMed
Zhang J, Varas F, Stadtfeld M, Heck S, Faust N, Graf T: CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation. Exp Hematol. 2007 Mar;35(3):490-499.
PubMed
Debili N, Robin C, Schiavon V, Letestu R, Pflumio F, Mitjavila-Garcia MT, Coulombel L, Vainchenker W: Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates. Blood. 2001 Apr 1;97(7):2023-30.
PubMed

Product specific references:

Wu XW., Chen Z., Bao CX., et al., 1989: Production and application of anti-human platelet GPIIb/IIIa monoclonal antibodies. National Medical J. of China. 69(8):427
PubMed
Variant
100 tests
PB-417-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Contact distributor
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