Anti-Hu CD41 PE-Cy™7

Anti-Hu CD41 PE-Cy™7
Regulatory status
RUO
Antigen
CD41
Clone
MEM-06
Format
PE-Cy™7
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T7-309-T100
Ask for delivery term HERE
275.00 USD
Variant
100 tests
T7-309-T100
Ask for delivery term HERE
275.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-06 reacts with an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
Workshop
HLDA X
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Leukocytes of patient suffering from LGL-type leukaemia.
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
Platelet GPIIb, Integrin alpha-IIb, GPalpha IIb, GTA
Antigen description
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Entrez Gene ID 3674
UniProt ID P08514
T7-309 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD41 (MEM-06) PE-Cy™7.

General references:

Mitjavila-Garcia MT, Cailleret M, Godin I, Nogueira MM, Cohen-Solal K, Schiavon V, Lecluse Y, Le Pesteur F, Lagrue AH, Vainchenker W: Expression of CD41 on hematopoietic progenitors derived from embryonic hematopoietic cells. Development. 2002 Apr;129(8):2003-13.
PubMed
Ferkowicz MJ, Starr M, Xie X, Li W, Johnson SA, Shelley WC, Morrison PR, Yoder MC: CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo. Development. 2003 Sep;130(18):4393-403.
PubMed
Zhang J, Varas F, Stadtfeld M, Heck S, Faust N, Graf T: CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation. Exp Hematol. 2007 Mar;35(3):490-499.
PubMed
Debili N, Robin C, Schiavon V, Letestu R, Pflumio F, Mitjavila-Garcia MT, Coulombel L, Vainchenker W: Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates. Blood. 2001 Apr 1;97(7):2023-30.
PubMed

Product specific references:

Belizaire RM, Prakash PS, Richter JR, Robinson BR, Edwards MJ, Caldwell CC, Lentsch AB, Pritts TA: Microparticles from stored red blood cells activate neutrophils and cause lung injury after hemorrhage and resuscitation. J Am Coll Surg. 2012 Apr;214(4):648-55
PubMed
Pagani G, Pereira JPV, Stoldt VR, Beck A, Scharf RE, Gohlke H: The human platelet antigen-1b (Pro33) variant of αIIbβ3 allosterically shifts the dynamic conformational equilibrium of this integrin toward the active state. J Biol Chem. 2018 Mar 30;293(13):4830-4844.
PubMed
Variant
100 tests
T7-309-T100
Ask for delivery term HERE
275.00 USD
Variant
100 tests
T7-309-T100
Ask for delivery term HERE
275.00 USD

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