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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD41/CD61 (PAC-1 epitope) Purified
Anti-Hu CD41/CD61 (PAC-1 epitope) Purified
Regulatory status
RUO
Antigen
CD41/CD61 (PAC-1 epitope)
Clone
PAC-1
Format
Purified
Reactivity
Human
Application
IP, FC (QC tested), ICC
Variant
0.1 mg
11-145-C100
In stock
150.00 USD
Variant
0.1 mg
11-145-C100
In stock
150.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgM kappa
Specificity
The mouse monoclonal antibody PAC-1 recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.
Application
IP, FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 2-4 μg/ml.
Reactivity
Human
Immunogen
Human platelets
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
platelet GPIIb, Integrin alpha-Iib, GPalpha IIb, GPIIb, GT, GP3A, platelet GPIIIa
Antigen description
CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.
Flow cytometry surface staining pattern of PHA stimulated human peripheral whole blood stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 μg/ml, GAM PE).
Flow cytometry multicolor staining pattern of human lymphocytes stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 μg/ml, GAM PE) and Hoechst 34580.
Separation of CD41/CD61 positive platelets (red-filled) from CD41/CD61 negative nucleated cells (black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated human peripheral whole blood stained using anti-human CD41/CD61 (PAC-1) purified antibody (concentration in sample 8 μg/ml, GAM PE).
Product specific references:
Shattil SJ, Hoxie JA, Cunningham M, Brass LF: Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation. J Biol Chem. 1985 Sep 15;260(20):11107-14.
PubMed
Shattil SJ, Cunningham M, Hoxie JA: Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood. 1987 Jul;70(1):307-15.
PubMed
Trikha M, Timar J, Lundy SK, Szekeres K, Cai Y, Porter AT, Honn KV: The high affinity alphaIIb beta3 integrin is involved in invasion of human melanoma cells. Cancer Res. 1997 Jun 15;57(12):2522-8.
PubMed
Bourne JH, Colicchia M, Di Y, Martin E, Slater A, Roumenina LT, Dimitrov JD, Watson SP, Rayes J: Heme induces human and mouse platelet activation through C-type-lectin-like receptor-2. Haematologica. 2021 Feb 1;106(2):626-629.
PubMed
Variant
0.1 mg
11-145-C100
In stock
150.00 USD
Variant
0.1 mg
11-145-C100
In stock
150.00 USD
Contact distributor
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SDS download
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