Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD40 APC

Anti-Hu CD40 APC

Regulatory status

RUO

Antigen

CD40

Clone

HB14

Format

APC

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-837-T100

In stock

242.00 USD

Variant

100 tests

1A-837-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody HB14 recognizes an extracellular epitope of CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.

Workshop

HLDA V

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

human B lymphoblastoid cell lines

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TNFRSF5, Bp50

Antigen description

CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.

Entrez Gene ID 958

UniProt ID P05540

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD40 (HB14) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human blood sample using anti-human CD40 (HB14) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD40 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD40 (HB14) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

General references:

Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF. CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions. J Exp Med. 1996 May 1;183(5):2209-18.
PubMed

Grewal IS, Flavell RA: The role of CD40 ligand in costimulation and T-cell activation. Immunol Rev. 1996 Oct;153:85-106.
PubMed

Pearson LL, Castle BE, Kehry MR: CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways. Int Immunol. 2001 Mar;13(3):273-83.
PubMed

Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB: Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells. Toxicol Appl Pharmacol. 2007 Dec 14
PubMed

Product specific references:

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Kansas GS, Tedder TF: Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent and independent adhesion in human B cells through a tyrosine kinase-dependent pathway. J Immunol. 1991 Dec 15;147(12):4094-102.
PubMed