Anti-Hu CD40 APC

Anti-Hu CD40 APC
Regulatory status
RUO
Antigen
CD40
Clone
HB14
Format
APC
Reactivity
Non-human primates, Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-837-T100
In stock
242.00 USD
Variant
100 tests
1A-837-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody HB14 recognizes an extracellular epitope of CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.
Workshop
HLDA V
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
human B lymphoblastoid cell lines
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TNFRSF5, Bp50
Antigen description
CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
Entrez Gene ID 958
UniProt ID P05540
1A-837_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD40 (HB14) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-837_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human blood sample using anti-human CD40 (HB14) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
1A-837_FC_Histogram
Separation of human CD40 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD40 (HB14) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

General references:

Oxenius A, Campbell KA, Maliszewski CR, Kishimoto T, Kikutani H, Hengartner H, Zinkernagel RM, Bachmann MF. CD40-CD40 ligand interactions are critical in T-B cooperation but not for other anti-viral CD4+ T cell functions. J Exp Med. 1996 May 1;183(5):2209-18.
PubMed
Grewal IS, Flavell RA: The role of CD40 ligand in costimulation and T-cell activation. Immunol Rev. 1996 Oct;153:85-106.
PubMed
Pearson LL, Castle BE, Kehry MR: CD40-mediated signaling in monocytic cells: up-regulation of tumor necrosis factor receptor-associated factor mRNAs and activation of mitogen-activated protein kinase signaling pathways. Int Immunol. 2001 Mar;13(3):273-83.
PubMed
Wu W, Alexis NE, Chen X, Bromberg PA, Peden DB: Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells. Toxicol Appl Pharmacol. 2007 Dec 14
PubMed

Product specific references:

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Kansas GS, Tedder TF: Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent and independent adhesion in human B cells through a tyrosine kinase-dependent pathway. J Immunol. 1991 Dec 15;147(12):4094-102.
PubMed
Variant
100 tests
1A-837-T100
In stock
242.00 USD
Variant
100 tests
1A-837-T100
In stock
242.00 USD

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