Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD4 Purified Low Endotoxin

Anti-Hu CD4 Purified Low Endotoxin

Anti-Hu CD4 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD4
Clone
MEM-115
Format
Purified Low Endotoxin
Reactivity
Human
Application
IP, FC (QC tested), FUNC
Variant
0.1 mg
12-203-C100
Out of stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The antibody MEM-115 recognizes an extracellular epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
Workshop
HLDA V: WS Code T T-CD04.09
Application
IP, FC (QC tested), FUNC
Application details
Functional application: The antibody MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions.
Immunoprecipitation: Excellent.
Flow cytometry: Recommended dilution: 3 μg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.
Reactivity
Human
Immunogen
Human thymocytes and T lymphocytes.
Other names
T4/Leu-3, L3T4
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
12-203 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD4 (MEM-115) purified antibody (low endotoxin), GAM-APC.

General references:

Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens. 1999 Jan;53(1):33-40.
PubMed

Product specific references:

Bosze S, Caccamo N, Majer Z, Mezo G, Dieli F, Hudecz F: In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis. Biopolymers. 2004;76(6):467-76.
PubMed
Singer II, Scott S, Kawka DW, Chin J, Daugherty BL, DeMartino JA, DiSalvo J, Gould SL, Lineberger JE, Malkowitz L, Miller MD, Mitnaul L, Siciliano SJ, Staruch MJ, Williams HR, Zweerink HJ, Springer MS: CCR5, CXCR4, and CD4 are clustered and closely apposed on microvilli of human macrophages and T cells.
PubMed
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
PubMed
Variant
0.1 mg
12-203-C100
Out of stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Contact distributor
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